Annals of Microbiology

, 57:609 | Cite as

Characterisation of cyclodextrin glucanotransferase fromBacillus circulans ATCC 21783 in terms of cyclodextrin production

  • Anna Vassileva
  • Nikolina Atanasova
  • Viara Ivanova
  • Pascal Dhulster
  • Alexandra Tonkova
Industrial Microbiology Original Articles

Abstract

Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) fromBacillus circulans ATCC 21783 was purified by ultrafiltration and a consecutive starch adsorption. Total enzyme yield of 75.5% and purification factor of 13.7 were achieved. CGTase was most active at 65°C, possessed two clearly revealed pH-optima at 6.0 and 8.6 and retained from 75 to 100% of its initial activity in a wide range of pH, between 5.0 and 11.0. The cyclising activity was enhanced by 1 mM CaCl2 or 4 mM CoCl2. The enzyme was thermostable up to 70°C, and 64% of the original activity remained at 70°C after 30 min heat treatment. Up to 41% conversion into cyclodextrins was obtained from 40 g l−1 starch without using any additives. This CGTase produced two types of cyclodextrins, beta and gamma, in a ratio 73:27 after 4 h reaction time at 65°C. This feature of the enzyme could be of interest for industrial cyclodextrin production.

Key words

Bacillus circulans ATCC 21783 cyclodextrin glucanotransferase cyclodextrins enzyme stability starch conversion 

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Copyright information

© University of Milan and Springer 2007

Authors and Affiliations

  • Anna Vassileva
    • 1
  • Nikolina Atanasova
    • 1
  • Viara Ivanova
    • 2
  • Pascal Dhulster
    • 3
  • Alexandra Tonkova
    • 1
  1. 1.Department of Extremophilic Bacteria, Institute of MicrobiologyBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Department of Organic Chemistry and MicrobiologyUniversity of Food TechnologiesPlovdivBulgaria
  3. 3.Laboratoire PROBIOGEM, UPRES-EA 1026Polytech’LilleVilleneuve d’AscqFrance

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