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Characterisation of cyclodextrin glucanotransferase fromBacillus circulans ATCC 21783 in terms of cyclodextrin production

  • Industrial Microbiology
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Abstract

Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) fromBacillus circulans ATCC 21783 was purified by ultrafiltration and a consecutive starch adsorption. Total enzyme yield of 75.5% and purification factor of 13.7 were achieved. CGTase was most active at 65°C, possessed two clearly revealed pH-optima at 6.0 and 8.6 and retained from 75 to 100% of its initial activity in a wide range of pH, between 5.0 and 11.0. The cyclising activity was enhanced by 1 mM CaCl2 or 4 mM CoCl2. The enzyme was thermostable up to 70°C, and 64% of the original activity remained at 70°C after 30 min heat treatment. Up to 41% conversion into cyclodextrins was obtained from 40 g l−1 starch without using any additives. This CGTase produced two types of cyclodextrins, beta and gamma, in a ratio 73:27 after 4 h reaction time at 65°C. This feature of the enzyme could be of interest for industrial cyclodextrin production.

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Authors and Affiliations

  1. Department of Extremophilic Bacteria, Institute of Microbiology, Bulgarian Academy of Sciences, 26, Acad. G. Bonchev str., 1113, Sofia, Bulgaria

    Anna Vassileva, Nikolina Atanasova & Alexandra Tonkova

  2. Department of Organic Chemistry and Microbiology, University of Food Technologies, 26, Maritsa str., 4002, Plovdiv, Bulgaria

    Viara Ivanova

  3. Laboratoire PROBIOGEM, UPRES-EA 1026, Polytech’Lille, aile C, boulevard Paul Langevin, cite scientifique, 59655, Villeneuve d’Ascq, France

    Pascal Dhulster

Authors
  1. Anna Vassileva
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  2. Nikolina Atanasova
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  3. Viara Ivanova
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  4. Pascal Dhulster
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  5. Alexandra Tonkova
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Correspondence to Alexandra Tonkova.

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Vassileva, A., Atanasova, N., Ivanova, V. et al. Characterisation of cyclodextrin glucanotransferase fromBacillus circulans ATCC 21783 in terms of cyclodextrin production. Ann. Microbiol. 57, 609–615 (2007). https://doi.org/10.1007/BF03175362

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  • DOI: https://doi.org/10.1007/BF03175362

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