Abstract
The exposure of chicken peripheral blood mononuclear cells (PBMCs) to pathogen can induce a rapid change in both pro-inflammatory and anti-inflammatory cytokine gene expression. Chicken PBMCs were infected with avian pathogenicEscherichia coli (APEC) and the isogenicfimH mutants of APEC, PBMCs immune response was determined by semi-quantitative RT-PCR. The results demonstrated that PBMCs were expressed TLR1/6/10, TLR3, TLR4, TLR5 and TLR7. APEC and non pathogenicE. Coli strains were significantly (P<0.05) increased the expression of IL-1β, IL-8, IL-18 and TGF-β4 cytokines compared to the isogenicfimH mutants. In addition, the opsonized avian pathogenicE. Coli strains were significantly (P<0.05) increased the expression of cytokines compared to the non-opsonized strains, whereas the opsonized avian non-pathogenicE. Coli strain was significantly (P < 0.05) decreased the expression of cytokines compared to the non-opsonized strain. PBMCs are important in APEC infection, the increase in cytokines expression may play important role in the regulation of immune response.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abbas A.K., Lichtman A.H. (2003). Immunity to microbes. In: Melley J., Ed., Cellular and Molecular Immunology. Elsevier Science, Philadelphia, pp. 345–366.
Adams S.C., Xing Z., Cardona C.J. (2008). Differing cytokine expression profiles in chicken and duck mononuclear cells in response to a duck-origin Avian Influenza Virus. FASEB J., 22: 561.
Akira S., Uematsu S., Takeuchi O. (2006). Pathogen recognition and innate immunity. Cell, 124: 783–801.
Alexopoulou L., Czopik A., Medzhitov R., Flavell R.A. (2001). Recognition of double-stranded RNA and activation of NFkB by Toll-like receptor 3. Nature, 413: 732–738.
Andersen-Nissen E., Hawn T.R., Smith K.D., Nachman A., Lampano A.E., Uematsu S., Akira S., Aderem A. (2007). Cutting edge: TLR5 −/− mice are more susceptible toEscherichia coli urinary tract infection.Immunology, 178: 4717–4720.
Arne P., Marc D., Bree A., Schouler C., Dho-Moulin M. (2000). Increased tracheal colonization in chickens without impairing pathogenic properties of avian pathogenicEscherichia coli MT78 with afimH deletion. Avian Dis., 44: 343–355.
Dasari P., Nicholson I.C., Hodge G., Dandie G.W., Zola H. (2005). Expression of toll-like receptors on B lymphocytes. Cellul. Immunol., 236: 140–145.
Fresno M., Kopf M., Rivas L. (1997). Cytokine and infectious diseases. Immunol. Today, 18: 56–58.
Fukui A., Inoue N., Matsumoto M., Nomura M., Yamada K., Matsuda Y. (2003). Molecular cloning and functional characterization of chicken Toll like receptors. J. Biol. Chem., 276: 143–149.
Gronlund U., Johannisson A., Persson W.K. (2006). Changes in blood and milk lymphocyte sub-populations during acute and chronic phases ofStaphylococcus aureus induced bovine mastitis. Res. Vet. Sci., 80: 147–154.
Harris G., Kuoless R., Chen W. (2006). Role of Toll-like receptors in health and diseases of gastrointestinal tract. World Gastrointestinal, 12: 2149–2160.
He S.F., Yuan Z.W., Zhu G.Q. (2008). Construction and characterization of type1 fimbriaefimH deletion mutant from avian pathogenicEscherichia coli. Acta Microbiologica Sinica, 48: 1–5.
Heil F., Hemmi H., Hochrein H., Ampenberger F., Kirschning C., Akira S. (2004). Species-specific recognition of single-stranded RNA via Toll-like receptor 7 and 8. Science, 303: 1526–1529.
Hornick D.B., Allen B.L., Horn M.A., Clegg S. (1991). Fimbrial types among respiratory isolates belonging to the family Enterobacteriaceae. Clin. Microbiol., 29: 1795–1800.
Iqbal M., Philbin V.J., Smith A.L. (2005). Expression patterns of chicken Toll-like receptor mRNA in tissues, immune cell subsets and cell lines. Vet. Immunol. Immunopath., 104: 117–127.
Kaiser P., Rothwell L., Galyovl E.E., Barrow P.A., Burnside J., Wigley P. (2000). Differential cytokine expression in avian cells in response to invasion bySalmonella typhimurium, Salmonella enteritidis andSalmonella gallinarum. Microbiology, 146: 3217–3226.
Kaiser M.G., Cheeseman J.H., Kaiser P., Lamont S.J. (2006). Cytokine expression in chicken peripheral blood mononuclear cells afterin vitro exposure toSalmonella enterica serovar Enteritidis. Poultry Sci., 85: 1907–1911
Kim W.K., Cho H.J., Ryu S.I., Hwang H.R., Kim D.H., Ryu H.Y., Chung J.W., Kim T.Y., Park B. C., Bae K. H., Ko Y., Lee S.C. (2008). Comparative proteomic analysis of peripheral blood mononuclear cells from atopic dermatitis patients and healthy donors. BMB Reports, 41: 597–603.
Kogut M.H. (2000). Cytokines and prevention of infectious diseases in poultry: a review. Avian Pathol., 29: 395–404.
Kogut M.H., Rothwell L., Kaiser P. (2005a). IFN gam priming of chicken heterophils upregulates the expression of proinflammatory and Th1 cytokine mRNA following receptor-mediated phagocytosis ofSalmonella enteric a serovar Enteritidis. J. Interferon Cytokine Res., 25: 73–81.
Kogut M.H., Iqbal M., He H., Philbin V., Kaiser P., Smith A. (2005b). Expression and function of Toll-like receptors in chicken heterophils. Dev. Comp. Immunol., 29: 791–807.
Krogfelt K.A., Bergmans H., Klemm P. (1990). Direct evidence that the FimH protein is the mannose-specific adhesin ofEscherichia coli type 1 fimbriae. Infect. Immun., 58: 1995–1998.
La Ragione R.M., Woodward M.J. (2002). Virulence factors ofEscherichia coli serotypes associated with avian colisepticaemia. Res. Vet. Sci., 73: 27–35.
Marc D., Arne P., Bree A., Dho-Moulin M. (1998). Colonization ability and pathogenic properties of afim_mutant of an avian strain ofEscherichia coli. Res. Microbiol., 149: 473–485.
Mellata M., Dho-Moulin M., Dozois C.M., Curtiss R., Lehoux B., Fairbrother J.M. (2003). Role of avian pathogenicEscherichia coli virulence factors in bacterial interaction with chicken heterophils and macrophages. Infect. Immun., 71: 494–503.
Moser B., Wolf M., Walz A., Loetscher P. (2004). Chemokines: multiple levels of leukocyte migration control. Trends Immunol., 25: 75–84.
Orskov F., Orskov I. (1992).Escherichia coli serotyping and disease in man and animals. Can. J. Microbiol., 38: 699–704.
Pourbakhsh S.A., Boulianne M., Martineau-Doize B., Dozois C.M., Desautels C., Fairbrother J.M. (1997). Dynamics ofEscherichia coli infection in experimentally inoculated chickens. Avian Dis., 41: 221–233.
Swaggerty C.L., Kogut M.H., Ferro P.J., Rothwell L., Pevzner I.Y., Kaiser P. (2004). Differential cytokine mRNA expression in heterophils isolated from Salmonella-resistant and -susceptible chickens. Immunology, 113: 139–148.
Zarember K.A., Godowski P.J. (2002). Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines. J. Immunol., 168: 554–561.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Musa, H.H., Wu, S.L., Zhu, C.H. et al. Immune response of peripheral blood mononuclear cells to avian pathogenicEscherichia coli . Ann. Microbiol. 59, 587–592 (2009). https://doi.org/10.1007/BF03175150
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03175150