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Evaluation of oil recovery by rhamnolipid produced with isolated strain from Iranian oil wells


A rhamnolipid producing bacterium,Pseudomonas aeruginosa HR was isolated from south of Iranian oil wells. In the previous study, effects of main factors of cultivation on production of biosurfactant by this strain were evaluated and their optimized values were determined. In the present paper, the ability of produced biosurfactant in oil recovery was investigated. For this purpose, its affect on enhanced oil recovery in sand pack and core holder obtained from oil wells was evaluated. It was found that the maximum oil recovery in saturated sand pack column and in saturated core holder of crude oil were 23.6 and 64%, respectively. Then, in core test mathematical model was proposed by considering interactions between given parameters. Furthermore, dependence of residual oil recovery on the process of core washing during injection of biosurfactant was investigated. Finally, capillary desaturation curve was compared with core flood data.

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Correspondence to Hamid Rashedi.

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Eskandari, S., Rashedi, H., Ziaie-Shirkolaee, Y. et al. Evaluation of oil recovery by rhamnolipid produced with isolated strain from Iranian oil wells. Ann. Microbiol. 59, 573 (2009).

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Key words

  • biosurfactant
  • Pseudomonas aeruginosa
  • rhamnolipid
  • MEOR
  • oil wells