Abstract
A strain named DS9 excreting organic solvent-stable lipase was screened and later identified asBacillus subtilis based on its phenotypes, biochemical test, and 16S rRNA gene sequence. Strain DS9 grows well on the medium with 10% (v/v) organic solvent with log P values equal to or above 2.5. The organic solvent-tolerant lipase excreted by strain DS9 had a wider tolerance for organic solvents. The relative activity of the lipase was above 60% at 37 °C, 200 rpm, 30 min in the present of 25% (v/v) organic solvents such as 1-butanol, hexanol, benzene, and toluene. The lipase was not only stable but also activated by n-hexane, xylene, heptane, isooctane, and n-decane. The optimal pH and temperature were 8.0 and 40 °C, respectively. Both the organic solvent-tolerant microorganism and the organic solvent-stable lipase produced by this strain could be used as a biocatalyst for application in non-aqueous biocatalysis.
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Liu, S., Fang, Y., Xu, W. et al. Screening and identification of a novel organic solvent-stable lipase producer. Ann. Microbiol. 59, 539–543 (2009). https://doi.org/10.1007/BF03175143
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DOI: https://doi.org/10.1007/BF03175143