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Isolation and characterization of cheese spoiler yeast isolated from Turkish white cheeses

  • Food Microbiology
  • Original Articles
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Abstract

Ninety-two yeast strains causing spoilage on different kinds of Turkish white cheeses were isolated and identified on the basis of their physiological and morphological properties. The identified isolates represented 12 species belonging to 8 genera. The most prevalent isolates belonged to the speciesDebaryomyces hansenii (32.6%),Kluyveromyces marxianus (18.5%) andYarrowia lipolytica (17.4%). Other genera encountered werePichia, Torulaspora, Candida, Williopsis, and Galactomyces. They were genotypically characterized with PCR amplification of the species-specific internally transcribed spacer (ITS) of 5.8S ribosomal DNA (ITS-PCR). ITS-PCR of 15 strains ofY. Lipolytica, 15 strains ofK. Marxianus and 25 strains ofD. Hansenii resulted in single fragments of 360, 740 and 640 bp, respectively. Proteolytic and lipolytic activity within yeast strains isolated from cheese was observed. Lipolytic and proteolytic organisms play an important role in the maturation of cheese and in the spoilage of dairy products. Usage of identification systems based on biochemical and physiological characteristics takes longer time and often results in misidentifications. In this study, false identification of 5 strains ofD. Hansenii, 2 strains ofK. Marxianus, 1 strain ofY. Lipolytica, 1 strain ofTorulaspora delbrüeckii, 1 strain ofPichia anamola and 1 strain ofCandida sake by using conventional methods confirmed the mentioned statement above. As a consequence, ITS-PCR was found to be more rapid, reliable, easy to perform and cost effective than biochemical approaches.

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References

  • Andrighetto C., Psomas E., Tzanetakis N., Suzzi G., Lombardi A. (2000). Randomly amplified polymorphic DNA (RAPD) PCR for the identification of yeasts isolated from dairy products. Lett. Appl. Microbiol., 30: 5–9.

    Article  CAS  PubMed  Google Scholar 

  • Atlas R.M., Parks L.C. (1997). Handbook of Microbiological Media, 2nd edn., CRC Pres, Boca Raton.

    Google Scholar 

  • Barnett J.A., Payne R.W., Yarrow D. (2000). Yeasts: Characteristics and Identification. Cambridge University Press, UK.

    Google Scholar 

  • Breuer U., Harms H. (2006).Debaryomyces hansenii — an extremophilic yeast with biotechnological potential. Yeast 23: 415–437.

    Article  CAS  PubMed  Google Scholar 

  • Deak T., Beuchat L.R. (1996). Handbook of Food Spoilage Yeasts. CRC Press, USA.

    Google Scholar 

  • Deak T., Chen J., Beuchat L.R. (2000). Molecular characterization ofYarrowia lipolytica andCandida zeylanoides isolated from poultry. Appl. Environ. Microbiol., 66: 4340–4344.

    Article  CAS  PubMed  Google Scholar 

  • Esteve-Zarzoso B., Belloch C., Uruburu F., Querol A. (1999). Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Int. J. Syst. Bacteriol., 49: 329–337.

    Article  CAS  PubMed  Google Scholar 

  • Fernandez-Espinar M.T., Esteve-Zarzoso B., Querol A., Barrio E. (2000). RFLP analysis of the ribosomal internal transcribed spacers and the 5.8S rRNA gene region of the genusSaccharomyces: a fast method for species identification and the differentiation of flor yeasts. Antonie van Leeuwenhoek, 78: 87–97.

    Article  CAS  Google Scholar 

  • Fleet G.H. (1990). Food spoilage yeasts. In: Spencer J.F.T., Spencer D.M., Eds, Yeast Technology. Springer, Berlin, pp. 124–166.

    Google Scholar 

  • Fujita S., Senda Y., Nakaguchi S., Hashimota T. (2001). Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains. J. Clin. Microbiol., 39: 3617–3622.

    Article  CAS  PubMed  Google Scholar 

  • Guillamon J.M., Sabate J., Barrio E., Cano J., Querol A. (1998). Rapid identification of wine yeast species based on RFLP analysis of the ribosomal internal transcribed spacer (ITS) region. Arch. Microbiol., 169: 387–392.

    Article  CAS  PubMed  Google Scholar 

  • Hayaloglu A.A, Guven M., Fox P.F. (2002). Microbiological biochemical and technological properties of Turkish White Cheese “Beyaz Peynir”. Int. Dairy J., 12: 635–648.

    Article  CAS  Google Scholar 

  • Jakobsen M., Norvhus J. (1996). Yeast and their possible beneficial and negative effects on the quality of dairy products. Int. Dairy J., 6: 755–768.

    Article  Google Scholar 

  • Leaw S.N., Chang C.H., Sun H.F., Barton R., Bouchara J.P., Chang C.T. (2006). Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions. J. Clin. Microbiol., 44: 693–699.

    Article  CAS  PubMed  Google Scholar 

  • Martin P.A., Florez A.B., Diaz T.M.L., Mayo B. (2007). Phenotypic and molecular identification of yeast species associated with Spanish blue-veined Cabrales cheese. Int. Dairy J., 17: 961–967.

    Article  CAS  Google Scholar 

  • Petersen K.M., Moller P.L., Jespensen L. (2001). DNA typing methods for differentiation ofDebaryomyces hansenii strains and other yeasts related to surface ripened cheeses. Int. J. Food Microbiol., 69: 11–24.

    Article  CAS  PubMed  Google Scholar 

  • Prillinger H., Molnar O., Eliskoses L.F., Lapondic K. (1999). Phenotypic and genotypic identification of yeasts from cheese. Antonie van Leeuwenhoek, 75: 267–283.

    Article  CAS  PubMed  Google Scholar 

  • Rapp P., Backhaus S. (1992). Formation of extracellular lipase by filamentous fungi, yeasts and bacteria. Enzyme Microb. Technol., 14: 938–943.

    Article  CAS  Google Scholar 

  • Roostita R., Fleet G.H. (1996). The occurrence and growth of yeast in Camembert and blue-veined cheese. Int. J. Food Microbiol., 28: 393–404.

    Article  CAS  PubMed  Google Scholar 

  • Romero P., Patino B., Quiros M., Gonzales-Jaen M.T., Valderrama M.J., de Silaniz M.I., Peinado J.M. (2005). Differential detection ofDebaryomyces hansenii isolated from intermediate-moisture foods by PCR-RFLP of the IGS region of rDNA. FEMS Yeast Res., 5: 455–461.

    Article  CAS  PubMed  Google Scholar 

  • Strauss M.L.A., Jolly N.P., Lambrechts M.G., Van Rensburg P. (2001). Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts. J. Appl. Microbiol., 91: 182–190.

    Article  CAS  PubMed  Google Scholar 

  • Vasdinyei R., Deak T. (2003). Characterization of yeast isolates originating from Hungarian dairy using traditional and molecular identification techniques. Int. J. Food Microbiol., 86: 123–130.

    Article  CAS  PubMed  Google Scholar 

  • Westall S., Filtenborg O. (1998). Spoilage yeasts of decorated soft cheese packed in modified atmosphere. Food Microbiol., 15: 243–249.

    Article  Google Scholar 

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Authors and Affiliations

  1. Ege University, Faculty of Science, Biology Department, Basic and Industrial Microbiology Section, 35100, Izmir, Turkey

    H. Tansel Yalcin & Fusun B. Ucar

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  1. H. Tansel Yalcin
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  2. Fusun B. Ucar
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Correspondence to H. Tansel Yalcin.

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Yalcin, H.T., Ucar, F.B. Isolation and characterization of cheese spoiler yeast isolated from Turkish white cheeses. Ann. Microbiol. 59, 477–483 (2009). https://doi.org/10.1007/BF03175134

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  • DOI: https://doi.org/10.1007/BF03175134

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