Skip to main content
Log in

In vitro degradation of sugarcane bagasse based ruminant rations using anaerobic fungi

  • Ecological and Environmental Microbiology
  • Original Articles
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

In present study thein vitro degradation of sugarcane bagasse and total mixed ration (i.e. sugarcane bagasse, wheat straw, and concentrate mixture in the ratio of 20∶30∶50) were tested using anaerobic rumen fungiNeocallimastix spp. GR1 andPiromyces spp. WNG-12. These cultures produced hydrolytic enzymes (CMCase, FPCase and xylanase)and increased thein vitro digestibility of dry matter, neutral detergent fibre, and acid detergent fibre of bagasse and total mixed rations when compared with control.Neocallimastix spp. GR1 andPiromyces spp. WNG-12 also increased total volatile fatty acids by degradation of sugarcane bagasse and total mixed ration. Based on results obtained, it may be stated thatNeocallimastix spp. GR1 may improve the nutritive value of high fibrous based diets.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • AOAC — Association of Official Analytical Chemists (2000). Official Methods of Analysis. International Volume I, 17th edn., Gaithersburg Maryland USA.

  • Barnett A.J.G., Reid R.L. (1957). Studies on production of volatile fatty acids from grass by rumen liquid in an artificial rumen. J. Agric. Sci. (Camb), 48: 315–321.

    Article  CAS  Google Scholar 

  • Dayananda T.L., Nagpal R., Puniya A.K., Sehgal J.P., Singh K. (2007).In-vitro degradation of urea-NH3 treated wheat straw using anaerobic ruminal fungi. J. Anim. Feed Sci., 16: 484–489.

    Google Scholar 

  • Dey A., Sehgal J.P., Puniya A.K., Singh K. (2004). Influence of an anaerobic fungal culture (Orpinomyces spp.) administration on growth rate ruminal fermentation and nutrient digestion in calves. Asian-Aust. J. Anim. Sci., 17: 820–824.

    Google Scholar 

  • Edwards J.E., Kingston-Smith A.H., Jimenez H.R., Huws S.A., Skot K.P., Griffith G.W., McEwan N.R., Theodorou M.K. (2008). Dynamics of initial colonization of nonconserved perennial ryegrass by anaerobic fungi in the bovine rumen. FEMS Microbiol. Ecol., 66 (3): 537–545.

    Article  CAS  PubMed  Google Scholar 

  • Goering H.K., VanSoest P.J. (1970). Forage Fibre Analysis Agricultural Handbook No. 379, USDA, Washington DC.

    Google Scholar 

  • Ha J.K., Lee S.S., Kim C.H., Choi Y.J., Min H.K. (1994). Effect of fungal inoculation on ruminal fermentation characteristics enzyme activities and nutrient-digestion in sheep. Proc. Soc. Nutr. Physiol., 3: 197.

    Google Scholar 

  • Joblin K.N. (1981). Isolation enumeration and maintenance of rumen anaerobic fungi in roll tubes. Appl. Environ. Microbiol., 42: 1119–1122.

    PubMed  Google Scholar 

  • Kawaminami T., Izuka H. (1970). Studies on xylanase from microorganisms. IV. Action of xylanase ofChaetomium trilaterate strain No. 2264 upon xylan. J. Ferment. Technol., 48: 161–168.

    CAS  Google Scholar 

  • Lee S.S., Ha J.K., Cheng K.J. (2000). Influence of an anaerobic fungal culture administration onin vivo ruminal fermentation and nutrient digestion. Anim. Feed Sci. Technol., 88: 201–217.

    Article  Google Scholar 

  • Mandels M., Andreotti R., Roche C. (1976).Measurement of sac-charifying cellulase. Biotechnol. Bioeng. Symp., 6: 21–23.

    CAS  PubMed  Google Scholar 

  • Manikumar B., Puniya A.K., Singh K., Sehgal J.P. (2004).In vitro degradation of cell wall and digestibility of cereal straws treated with anaerobic ruminal fungi. Indian J. Exp. Biol., 42: 636–638.

    Google Scholar 

  • McAllister T.A., Bae H.D., Yanke L.J., Cheng K.J., Muir A. (1994). Effect of condensed tannins from birdsfoot trefoil on endoglucanase activity and the digestion of cellulose filter paper by ruminal fungi. Can. J. Microbiol., 40: 298–305.

    Article  CAS  PubMed  Google Scholar 

  • McDougall E.I. (1948). Studies on ruminant saliva. I. The composition and output of sheeps’ saliva. Biochem. J., 43: 99–109.

    CAS  Google Scholar 

  • Miller G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem., 31: 426.

    Article  CAS  Google Scholar 

  • Paul S.S., Kamra D.N., Sastry V.R.B., Agarwal N. (2004). Effect of administration of an anaerobic gut fungus isolated from wild blue bull to buffaloes onin-vivo ruminal fermentation and digestion of nutrients. Anim. Feed Sci. Tech., 115: 143–157.

    Article  Google Scholar 

  • Reddy G.V.N., Reddy K.J., Nagalakshmi D. (2002). Effect of expander-extruder processed complete diet containing sugarcane bagasse on growth and nutrient utilization in Ongole bull calves. Indian J. Anim. Sci., 72: 406–409.

    Google Scholar 

  • Reddy M.R., Chandrasekharaiah M., Govindaiah T., Reddy G.V.N. (1993). Effect of physical processing on the nutritive value of sugarcane bagasse in goat and sheep. Small Ruminant Res., 10: 25–30.

    Article  Google Scholar 

  • Robertson J.B., VanSoest P.J. (1977). Dietary fibre estimation in concentrate feedstuffs. J. Anim. Sci., 45: 254.

    Google Scholar 

  • Sehgal J.P., Jit D., Puniya A.K., Singh K. (2008). Influence of anaerobic fungal administration on growth, rumen fermentation and nutrient digestion in female buffalo calves. J. Anim. Feed Sci., 17: 510–518.

    Google Scholar 

  • Snedecor G.W., Cochran W.G. (1980). Statistical Methods 7th edn., The Iowa State University Press, Iowa USA.

    Google Scholar 

  • Srinivasan K., Murakami M., Nakashimada Y., Nishio N. (2001). Efficient production of cellulolytic and xylanolytic enzymes by the rumen anaerobic fungus,Neocallimastix frontalis in a repeated batch culture. J. Biosci. Bioeng., 91: 153–158.

    Article  CAS  PubMed  Google Scholar 

  • Thareja A., Puniya A.K., Goel G., Nagpal R., Sehgal J.P., Singh P., Singh K. (2006).In vitro degradation of wheat straw by anaerobic fungi from small ruminants. Arch. Anim. Nutr., 60: 412–417.

    Article  CAS  PubMed  Google Scholar 

  • Tilley J.M.A., Terry R.A. (1963). A two stage technique for thein vitro digestion of forage crops. J. British Grass. Soc., 18: 104–111.

    Article  CAS  Google Scholar 

  • Trinci A.P.J., Davies D.R., Gull K., Lawrence M.I., Nielsen B.B., Rickers A., Theodorou M.K. (1994). Anaerobic fungi in herbivorous animals. Mycol. Res., 98: 129–152.

    Article  Google Scholar 

  • Tripathi V.K., Sehgal J.P., Puniya A.K., Kishan S. (2007a). Hydrolytic activities of anaerobic fungi isolated from wild blue bull (Boselaphus tragocamelus). Anaerobe, 13: 36–39.

    Article  CAS  PubMed  Google Scholar 

  • Tripathi V.K., Sehgal J.P., Puniya A.K., Singh K. (2007b). Effect of administration of anaerobic fungi isolated from cattle and wild blue bull (Boselaphus tragocamelus) on growth rate and fibre utilization in buffalo calves. Arch. Anim. Nutr., 61: 416–423.

    Article  CAS  PubMed  Google Scholar 

  • Zadrazil F., Puniya A.K. (1995). Studies on effect of particle size during solid state fermentation of sugarcane bagasse into animal feed using white rot fungi. Bioresource Technol., 54: 85–87.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Anil Kumar Puniya.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shelke, S.K., Chhabra, A., Puniya, A.K. et al. In vitro degradation of sugarcane bagasse based ruminant rations using anaerobic fungi. Ann. Microbiol. 59, 415–418 (2009). https://doi.org/10.1007/BF03175124

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03175124

Key words

Navigation