Annals of Microbiology

, 56:359 | Cite as

Antifungal activity of olive leaf (Olea Europaea L.) extracts from the Trilye Region of Turkey

  • Mihriban KorukluogluEmail author
  • Yasemin Sahan
  • Aycan Yigit
  • Reyhan Karakas
Physiology and Metabolism Original Articles


Antimicrobial properties of olive leaf extract on some yeast were examined in this study. Fresh olive leaf extracts were prepared using various solvents (water, ethanol, acetone, ethyl acetate) in Soxhlet apparatus. Antimicrobial effects of these extacts were tested againstSaccharomyces cerevisiae ATCC 9763,Schizosaccharomyces pombe, Saccharomyces uvarum, Candida oleophila, Metschnikowia fructicola andKloeckera apiculata. The antifungal activities of these extracts were tested by the disc diffusion assay, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). All extracts showed various degrees of antifungal effects with 10–28 μg/ml MIC, 20–48 μg/ml MFC and 1.5–9.3 mm inhibitory zone values against yeasts utilised, except water. The results indicated that the tested yeasts were sensitive to acetone and ethyl acetate extracts. It was determined thatSaccharomyces cerevisiae ATCC 9763 was the most resistant among the yeasts.

Key words

Olea europaea L. antifungal minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) 


  1. Arora D.S., Kaur J. (1999). Antimicrobial activity of spices. Int. J. Antimicrob. Agents, 12: 257–262.CrossRefPubMedGoogle Scholar
  2. Aziz N.H., Farag S.E., Mousa L.A., Abo-Zaid M.A. (1998). Comparative antibacterial and antifungal effects of some phenolic compounds. Microbios, 93: 43–54.PubMedGoogle Scholar
  3. Battinelli L., Daniele C., Cristiani G., Mazzanti G. (2006).In vitro antifungal and anti-elastase activity of some aliphatic aldehydes fromOlea europaea L. fruit. Phytomedicine, 13 (8): 558–563.CrossRefGoogle Scholar
  4. Benavente-Garcia O., Castillo J., Lorente J., Ortuno A., Del Rio J.A. (2000). Antioxidant activity of phenolics extracted fromOlea europaea L. leaves. Food Chem., 68: 457–462.CrossRefGoogle Scholar
  5. Bisignano G., Tomaino A., Lo Cascio R., Crisafi G., Uccella N., Saija A. (1999). On thein-vitro antimicrobial activity of oleuropein and hydroxytyrosol. J. Pharm. Pharmacol., 31: 971–974.CrossRefGoogle Scholar
  6. Evans D.G., Everis K., Betts G.D. (2004). Use of survival analysis and Classification and Regression Trees to model the growth/no growth boundary of spoilage yeasts as affected by alcohol, pH, sucrose, sorbate and temperature. Int. J. Food Microbiol., 92: 55–67.CrossRefPubMedGoogle Scholar
  7. Fleming H.P., Walter W.H.J., Etchells J.L. (1973). Antimicrobial properties of oleuropein and products of its hydrolysis from green olives. Appl. Microbiol., 26: 777–782.PubMedGoogle Scholar
  8. Gourama H., Bullerman L.B. (1987). Effect of oleuropein on growth and aflatoxin production byAspergillus parasiticus. Lebensm. Wiss. U. Technol., 20: 226–228.Google Scholar
  9. Gourama H., Letutour B., Tantaoui Elaraki, A., Benbya M., Bullerman L.B. (1989). Effects of oleuropein, tyrosol and caffeic acid on the growth of mold isolated from olives. J. Food Protect., 52: 264–266.Google Scholar
  10. Ismail S.A.S., Deaka T., Abd El-Rahman H.A., Yassien M.A.M., Beuchat L.R. (2000). Presence and changes in populations of yeasts on raw and processed poultry products stored at refrigeration temperature. Int. J. Food Microbiol., 62: 113–121.CrossRefPubMedGoogle Scholar
  11. Juven B., Samish Z., Henis Y. (1968). Identification and oleuropein as a natural inhibitor of lactic fermentation of green olives. Israel J. Agric. Res., 18:137–138.Google Scholar
  12. Juven B., Henis Y. (1970). Studies on antimicrobial activity of olive phenolic compounds. J. Appl. Bacteriol., 33: 721–732.PubMedGoogle Scholar
  13. Karaman I., Sahin F., Gulluce M., Ogutcu H., Sengul M., Adiguzel A. (2003). Antimicrobial activity of aqueous and methanol extracts ofJuniperus oxycedrus L. J. Ethnopharmacol., 85: 231–235.CrossRefPubMedGoogle Scholar
  14. Korukluoglu M., Sahan Y., Yigit A., Tumay Ozer E., Gucer S. (2004).In vitro antibacterial activity of olive leaf (Olea europaea L.) extracts and their chemical characterization. Kusadas-Aydin, 4th Aegean Analytical Chemistry Days, Turkey.Google Scholar
  15. Khayyal, M.T., El-Ghazaly, M.A., Abdallah, D.M., Nassar, N.N., Okpanyi, S.N., Kreuter, M.H. (2002). Blood pressure lowering effect of an olive leaf extract (Olea europaea) in L-NAME induced hypertension in rats. Arzneimittelforschung, 52(11): 797–802.PubMedGoogle Scholar
  16. Le Tutour B., Guedon D. (1992). Antioxidative activities ofOlea europaea leaves and related phenolic compounds. Phytochemistry, 31: 173–1178.Google Scholar
  17. Loureiro V., Queroly A. (1999). The prevalence and control of spoilage yeasts in foods and beverages. Trends Food Sci. Technol., 10: 356–365.CrossRefGoogle Scholar
  18. Loureiro V., Malfeito-Ferreira M. (2003). Spoilage yeasts in the wine industry. Int. J. Food Microbiol., 86: 23–50.CrossRefPubMedGoogle Scholar
  19. Mahjoub A., Bullerman L.B. (1987). Effects of nutrients and inhibition in olives on aflatoxigenic molds. J. Food Protect., 50: 959–963.Google Scholar
  20. Markin D., Duek L., Berdicevsky I. (2003). In vitro antimicrobial activity of olive leaves. Mycoses, 46: 132–136.CrossRefPubMedGoogle Scholar
  21. McDonald S., Prenzler P.D., Antolovich M., Robards K. (2001). Phenolic content and antioxidant activity of olive extracts. Food Chem., 73: 73–84.CrossRefGoogle Scholar
  22. Micol V., Caturla N., Pèrez-Fons L., Más V., Pèrez Estepa A. (2005). The olive leaf extract exhibits antiviral activity against viral haemorhagic septicaemia rhabdovirus (VHSV). Antiviral Res., 66: 129–136.CrossRefPubMedGoogle Scholar
  23. Portillo A., Vila R., Freixa B., Ferro E., Parella T., Casanova J., Canigueral S. (2005). Antifungal sesquiterpene from the root ofVernonnanthura tweedieana. J. Ethnopharmacol., 97: 49–52.CrossRefPubMedGoogle Scholar
  24. Ranalli A., Contento S., Lucera L., Di Febo M., Marchegiani D., Di Fonzo V. (2006). Factors affecting the contents of iridoid oleuropein in olive leaves (Olea europaea L.). J. Agric. Food Chem, 54: 434–440.CrossRefPubMedGoogle Scholar
  25. Rasooli I., Abyaneh M.R. (2004). Inhibitory effects of thyme oils on growth and aflatoxin production byAspergillus parasiticus. Food Contr., 15: 479–483.CrossRefGoogle Scholar
  26. Rauha J.P., Remes S., Heinonen M., Hopia A., Kahkonen M., Kujala T., Pihlaja K., Vuorela H., Vuorela P. (2000). Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int. J. Food Microbiol., 56: 3–12.CrossRefPubMedGoogle Scholar
  27. Sagdic O., Kuscu A., Ozcan M., Ozcelik S. (2002). Effects of Turkish spice extracts at various concentrations on the growth ofEscherichia coli O157:H7. Food Microbiol., 19: 479–480.Google Scholar
  28. Sahin F., Güllüce M., Daferera D., Sokmen A., Sokmen M., Polissiou M. Agar G., Ozer H. (2004). Biological activities of the essential oils and methanol extract ofOriganum vulgare ssp.vulgare in the Eastern Anatolia region of Turkey. Food Contr., 15: 549–557.CrossRefGoogle Scholar
  29. Soler-Rivas C., Espin J.C., Wichers H.J. (2000). Oleuropein and related compounds. J. Sci. Food Agric., 80: 1013–1023.CrossRefGoogle Scholar
  30. Sousa A., Ferreira I.C.F.R., Calhelha R., Andrade P.B., Valentao P., Seabra R., Estevinho L., Bento A., Pereira J.A. (2006). Phenolics and antimicrobial activity of traditional stoned table olives ‘alcaparra’. Bioorg. Med. Chem. (In press).Google Scholar
  31. Tassou C.C., Nychas G.J.E. (1995). Inhibition ofSalmonella enteriditis by oleuropein in growth broth and in a model food system. Lett. Appl. Microbiol., 20: 120–124.CrossRefPubMedGoogle Scholar
  32. Tranter H.S., Tassou C.C., Nychas G.J.E. (1993). The effect of the olive phenolic compound, oleuropein, on growth and enterotoxin B production byStaphylococcus aureus. J. Appl. Bacteriol., 74: 253–259.PubMedGoogle Scholar
  33. Tuck K.L., Hayball P.J. (2002). Major phenolic compounds in olive oil: metabolism and health effects. J. Nutr. Biochem., 13: 636–644.CrossRefPubMedGoogle Scholar
  34. Yigit A., Sahan Y., Korukluoglu M. (2001). Antimicrobial substances found in olive leaves and olive. 2nd International Altinoluk “Antandros” Olive Busines Symposium, Turkey, pp. 139–147.Google Scholar
  35. Yin M., Tsao S. (1999). Inhibitory effect of sevenAllium plants upon threeAspergillus species. Int. J. Food Microbiol., 49: 49–56.CrossRefPubMedGoogle Scholar
  36. Zarzuelo A. (1991). Vasodilator effect of olive leaf. Planta Med., 57: 417–419.CrossRefPubMedGoogle Scholar

Copyright information

© University of Milan and Springer 2006

Authors and Affiliations

  • Mihriban Korukluoglu
    • 1
    Email author
  • Yasemin Sahan
    • 1
  • Aycan Yigit
    • 1
  • Reyhan Karakas
    • 2
  1. 1.Department of Food Engineering, Faculty of AgricultureUludag UniversityBursaTurkey
  2. 2.Susurluk CollegeBalikesir UniversitySusurluk, BalikesirTurkey

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