Annals of Microbiology

, Volume 56, Issue 4, pp 353–358 | Cite as

Chemical composition and antifungal effect of anise (Pimpinella anisum L.) fruit oil at ripening stage

  • Mehmet Musa ÖzcanEmail author
  • Jean Claude Chalchat
Physiology and Metabolism Original Articles


The composition of the essential oil ofPimpinella anisum L fruit is determined by GC and GC-MS. The volatile oil content obtained by hydrodistillation was 1.91%. Ten compounds representing 98.3% of the oil was identified. The main constituents of he oil obtained from dried fruits were trans-a nethole (93.9%) and estragole (2.4%). The olfactorially valuable constituents that were found with concentration higher than 0.06% were (E)-methyeugenol, α-cuparene, α-himachalene, β-bisabolene, p-anisaldehyde and cis-anethole. Also, the different concentrations of anise oil exerted varying levels of inhibitory effects on the mycelial growth off/ternaria alternata, Aspergillus niger andAspergillus parasiticus used in experimental. The results showed that the most effected fungus from anise oil wasA. parasiticus, which is followed byA. niger andA. alternata. Individual of this plant oil may provide a useful to achive adequate shelf-life of foods.

Key words

anise Pimpinella anisum essential oil composition trans-anethole fungi inhibitory effect 


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  1. Aboutabl E.A., Sokar N.M., Megid R.M.A. (1995). Composition and antimicrobial activity ofOtostegia fruticosa Forssk. J. Essent. Oil Res., 7: 299–303.Google Scholar
  2. Adams R. (2001). Essential Oil Components by Quadrupole GC/MS Allured Publishing Corp., Carol Stream, IL, USA.Google Scholar
  3. Agrios G.N. (1997). Plant Pathology, 4th edn., Academic Pres, London.Google Scholar
  4. Akgül A., Kivanç M., Sert S. (1991). Effect of carvacrol on growth and toxin production byAspergillus flavus andAspergillus parasiticus. Sci. Aliment., 11: 361–370.Google Scholar
  5. Ashraf M., Siddiqui M.A., Bhatty M.K. (1980). Studies on the essential oils of the Pakistan species of the family Umbelliferae. Pakistan J. Sci. Ind. Res., 23: 211–212.Google Scholar
  6. Askari F., Sefidkon F., Mirza M. (1998). Quantitative and qualitative study of components in essential oil ofPimpinella anisum L. Journal of Pajouhes-Va-Sazandegi, 38: 70–77.Google Scholar
  7. Askari F., Sefidkon F., Mozafarian V. (2005). Essential oil composition ofPimpinella aurea D.C. from Iran. Flavour Fragr. J., 20: 115–117.CrossRefGoogle Scholar
  8. Anonymous (2005a). Available in: http// Scholar
  9. Azzouz M.A, Bullerman L.B. (1982). Comparative antimycotic effects of selected herbs, spices, plant components and commercial antifungal agents. J. Food Protect., 45: 1298–1301.Google Scholar
  10. Başer K.H.C., Özek T. (1996). Essential oil ofPimpinella aromatica Bieb. from Turkey. J. Essent. Oil Res., 8: 463–464.Google Scholar
  11. Baytop T. (1984). Treatment with Plants in Turkey, Istanbul Univ. Publ. Nu. 3255, Istanbul Turkey (in Turkish).Google Scholar
  12. Bown D. (1995). Encyclopedia of Herbs and their Uses. Dorling Kindersley, London.Google Scholar
  13. Boyraz N., Özcan M. (2006). Inhibition of phytopathogenic fungi by essential oil, hydrosol, ground material and extract of summer savor (Satureja hortensis L.) growing wild in Turkey. Int. J. Food Microbiol., 107: 238–242.CrossRefPubMedGoogle Scholar
  14. Caccioni D.R.L., Guizzardi M. (1998). Inhibition of germination and growth of fruit and vegetable postharvest pathogenic fungi by essential oil components. J. Essent. Oil Res., 6: 173–179.Google Scholar
  15. Chevallier A. (1996). The Encyclopedia of Medicinal Plants. Wolfe Publishing Ltd., London.Google Scholar
  16. Deans S.G., Svoboda K.P. (1990). The antimicrobial properties of marjoram (Origanum majorana L.) volatile oil. Flavour Fragr. J., 5: 187–190.CrossRefGoogle Scholar
  17. Dizdaroĝlu T., Balkan C. (1996). Anise: its production, marketing and constrains in Izmir province. Anadolu, 6: 36–53.Google Scholar
  18. Dorman H.J.D., Deans S.G. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology 88: 308–316.CrossRefPubMedGoogle Scholar
  19. Embong M.B., Hadzie D., Molnar S. (1977). Essential oil in Alberta anise oil (Pimpinella anisum L.). Can. J. Plant Sci., 59: 681–688.CrossRefGoogle Scholar
  20. Erkmen O., Özcan M. (2001). Antimicrobial activites of essential oils from Turkish spices. Eur. Food Res. Technol., 212 (6): 658–660.CrossRefGoogle Scholar
  21. Farag R.S., Daw Z.Y., Abo-Raya S.H. (1989). Influence of some spice essential oils onAspergillus parasiticus growth and production of aflatoxins in a synthetic medium. J. Food Protect., 54: 74–76.Google Scholar
  22. Fawcet C.H., Spencer D.M. (1970). Plant chemoteraphy with natural products. Ann. Rev. Phytopathol., 403–418.Google Scholar
  23. Hedrick U.P. (1972). Sturtevant’s Edible Plants of the World. Dover Publications.Google Scholar
  24. Ivanic R., Savin K., Robinson F.V. (1983). Essential oil fromPimpinella serbica fruits. Planta Med., 48 (1): 60–61.CrossRefPubMedGoogle Scholar
  25. Kubo I., Schilcher H., Philipsan J.D., Loew A. (1993). Anethol, a synergist of polygodial and wrburganal againstCandida albicans fungi. Acta Horticulture, 332: 191–197.Google Scholar
  26. Lawrence D.M. (1984). Progres in essential oils [Anise oil, Calamus oil, Cardamom oils]. Perfumer and Flavorist, 8: 63–65.Google Scholar
  27. Omidbaigi R., Hadjiakhoondi A., Saharkhiz M. (2003). Changes in content and chemical composition ofPimpinella anisum oil at various harvest time. J. Essent. Oil Bearing Plants, 6 (1): 46–50.Google Scholar
  28. Özcan M. (1998). Inhibitory effects of spice extracts on the growth ofAspergillus parasiticus NRRL 2999 strain. Z. Lebensm. Unters. Forsch. A, 207: 253–255.CrossRefGoogle Scholar
  29. Özcan M. (2003). Effect of essential oils of some plants used as thyme on the growth ofAspergillus parasiticus NRRL 2999 strain. J. Essent. Oil Bearing Plants 6(1): 55–59.Google Scholar
  30. Özcan M. (2005). Effect of spice hydrosols on the growth ofAspergillus parasiticus NRRL 2999 strain. J. Med. Food, 8 (2): 275–278.CrossRefPubMedGoogle Scholar
  31. Phillips R., Foy N. (1990). Herbs. Pan Books Ltd, London.Google Scholar
  32. Püskülcü H., Ikiz F. (1989). Introduction to Statistics. Bilgehan Press, Bornova-Izmir, Turkey.Google Scholar
  33. Riotte H. (1978). Companion Planting for Succesful Gardening. Garden Way, Vermont, USA.Google Scholar
  34. Rodrigues V.M., Rosa P.T.V., Marques M.O.M., Petenale A.S., Meireles M.A.A. (2003). Supercriticial extraction of essential oil from aniseed (Pimpinella anisum L.) using CO2 solubility, kinetics and composition data. J. Agric. Food Chem., 51: 1518–1523.CrossRefPubMedGoogle Scholar
  35. Singh S.P., Rao G.P., Upadyaya P.P. (1998). Fungitoxicity of essential oils of some aromatic plant against sugar cane pathogens. Sugar Cane, 2: 14–17.Google Scholar
  36. Sokoviç M., Tzakou O., Pitarokili D., Couladis M. (2002). Antifungal activities of selected aromatic plants growing wild in Greece. Nahrung-Food, 46 (5): 317–320.CrossRefGoogle Scholar
  37. Shelef L.A. (1983). Antimicrobial effects of spices. J. Food Sci., 6: 29–44.Google Scholar

Copyright information

© University of Milan and Springer 2006

Authors and Affiliations

  1. 1.Department of Food Engineering, Faculty of AgricultureUniversity of SelcukKonyaTurkey
  2. 2.Laboratoire de Chimie des Huiles EssentiellesUniversite Blaise Pascal de ClermontAubiere CedexFrance

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