Abstract
In this paper, we investigated the properties of trehalose-6- phosphate synthase (SfTps1) inSaccharomycopsis fibuligera sdu a high-trehalose-accumulating strain. The purified SfTps1 showed a band on Native-PAGE and SDS-PAGE of about 66 kDa. The optimal pH and temperature of the purified enzyme were 6.6 and 37 °C, respectively. The enzyme was activated by Ca2+, K+ and Mg2+, inhibited by Mn2+, Cu2+, Fe3+, Hg2+ and Co2+. Iodoacetic acid, EDTA and PMSF had inhibitory effect on the enzyme activity. Km values of the enzyme for glucose-6-phosphate and UDP-glucose were 38.6 mM and 9.3 mM, respectively. The effects of various stress conditions on SfTps1 activity and trehalose content in this strain were also studied. Neither the activation of SfTps1 nor the change in trehalose content was observed under stress exposure ofSaccharomycopsis fibuligera cells. Our results indicate that the SfTps1 protein and trehalose metabolism in response to stress conditions inSaccharomycopsis fibuligera clearly differ from that ofSaccharomyces cerevisiae and most of other eukaryotes.
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Liang, L., Chi, Z., Wang, X. et al. Properties of trehalose-6-phosphate synthase fromSaccharomycopsis fibuligera . Ann. Microbiol. 56, 241–245 (2006). https://doi.org/10.1007/BF03175012
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DOI: https://doi.org/10.1007/BF03175012