Abstract
Chlorpromazine (CPZ), a phenothiazine derivative, is a potent antipsychotic agent and imipramine (IP) is a widely used tricyclic antidepressant. The interaction between these molecules and erythrocyte membranes is of particular interest considering the role of these cells in the transport and release of these drugs at the central nervous system. In the present paper, we intend to study the effects of IP on erythrocyte membranes and to compare these effects with those of CPZ. Erythrocytes from adult Sprague-Dawley rats were incubated separately with different concentrations of IP or CPZ for 1 h at room temperature, fixed and stained by Giemsa. Changes in erythrocyte morphology were quantified by an image analysis system. The interaction of both drugs, CPZ and IP, with the erythrocyte membrane causes similar changes in cell shape. Increasing concentrations of both drugs induces the formation of stomatocytes, spherostomatocytes and spherocytes, because of an irreversible loss of area and volume, probably due to endovesiculation. Our results also show that the CPZ is more potent than IP.
Resumen
La clorpromazina (CPZ), un derivado de las fenotiazinas, es un potente agente antipsicótico y la imipramina (IP) es un antidepresivo tricíclico extensamente utilizado. La interacción entre estas moléculas y las membranas de los eritrocitos es de particular interés considerando la importancia de estas células en el transporte y liberación de estos fármacos en el sistema nervioso central. En este trabajo, nos proponemos estudiar los efectos de la IP en las membranas de los eritrocitos y comparar esos efectos con los de CPZ. Los eritrocitos de ratas Sprague-Dawley adultas fueron incubados por separado con varias concentraciones de IP o CPZ durante 1h a temperatura ambiente, fijados y teñidos con Giemsa. Los cambios en morfología de los eritrocitos fueron cuantificados mediante un sistema de análisis de imagen. La interacción de ambos productos, CPZ e IP, con la membrana de los eritrocitos provoca cambios similares en forma de la célula. El aumento de la concentración de ambos fármacos induce la formación de estomatocitos, esferoestomatocitos y esferocitos, debido a una pérdida irreversible de área y de volumen, probablemente debido a la endovesiculación, siendo la CPZ más potente que la IP.
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Ahyayauch, H., Gallego, M., Casis, O. et al. Changes in erythrocyte morphology induced by imipramine and chlorpromazine. J. Physiol. Biochem. 62, 199–205 (2006). https://doi.org/10.1007/BF03168469
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DOI: https://doi.org/10.1007/BF03168469