Abstract
Electrophoretic polypeptide profiles of normal human saliva differ markedly between different reports. Since both methodological variations and polymorphism may explain these differences, in this study we aimed to establish whether or not the salivary electrophoretic polypeptide profiles of subjects from a healthy population share discrete molecular features. To this end, parotid, submandibular/sublingual and whole salivas were collected separately from each of 40 young and 34 elderly clinically healthy adults and processed for SDS-polyacrylamide gel electrophoresis and Coomassie blue staining. Each type of glandular saliva displayed a different group of invariant (i.e. present in every subject) electrophoretic polypeptide bands while whole saliva showed a profile that reflected mostly the combined contribution of the major salivary glands. Some minor variant (i.e. absent in some subjects) bands were identified in each type of saliva. Regarding those interindividual variations, no ageor sex-dependence was appreciated. Altogether, these results demonstrate the occurrence of distinctive electrophoretic polypeptide patterns, in addition to some minor variations, for each type of normal saliva, thus providing a background for further populational studies on salivary polypeptide profiles.
Resumen
Los perfiles electroforéticos de la saliva humana normal publicados presentan marcadas diferencias. Puesto que estas diferencias podrían deberse a polimorfismo poblacional o a variaciones metodológicas, en el presente estudio pretendemos determinar si los perfiles polipeptídicos electroforéticos salivales de una población sana comparten rasgos moleculares. Con este propósito, se colectó saliva parotídea, saliva submandibular/sublingual y saliva total de 40 sujetos adultos jóvenes y 34 sujetos adultos mayores, clínicamente sanos. Las muestras de saliva fueron sometidas a fraccionamiento electroforético en geles de poliacrilamida-SDS y reveladas por tinción con azul de Coomassie. Cada tipo de saliva glandular presentó un grupo característico de bandas polipeptídicas invariables (presentes en todos los sujetos). En la saliva total se pudo observar un perfil que reflejaba principalmente la contribución proteica de las glándulas salivales mayores. En todos los tipos salivales analizados, se pudo observar algunos polipéptidos variables (ausentes en algunos sujetos). Estas variaciones interindividuales no se relacionaron con el sexo o la edad de los individuos. El conjunto de los resultados demuestra la existencia de patrones polipeptídicos distintivos para cada tipo salival junto con la presencia de algunos pocos rasgos variantes en condiciones de normalidad, lo que podría servir de base en futuros estudios poblacionales sobre perfiles polipeptídicos salivales.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Atkinson, J.C., Dawqes, C., Ericson, T., Fox, P.C., Gandara, B.K., Malamud, D.,et al. (1993):Ann. NY Acad. Sci.,694, 1–348.
Azen, E.A., Yu, P. (1984):Biochem. Genet.,22, 1–19.
Bramhall, S., Noack, N., Wu, M., Loewenberg, J. R. (1969):Anal. Biochem.,31, 146–148.
Becerra, L., Soares, R., Bruno, L., Siqueira, C., Oppenheim, F.G., Offner, G.D.,et al. (2003):Arch. Oral Biol.,48, 147–154.
Carlén, A., Eliasson, L., Aronsson, G., Birkhed, D. (2004):Arch. Oral Biol.,49, 177–181.
Chaushu, S., Yefenol, E., Becker, A., Shapira, J., Chaushu, G. (2002):J. Dent. Res.,81, 308–312.
Doods, M. W. J., Yeh, C-K., Johnson, D. A. (2000):Commun. Dent. Oral Epidemiol.,28, 373–381.
Durham, J. P., López-Solís, R. O. (1979):Anal. Biochem.,100, 98–99.
Ghafouri, B., Tagesson, C., Lindahl, M. (2003):Proteomics,3, 1003–1015.
Ghezzi, E.M., Ship, J.A. (2003):J. Dent. Res.,82, 844–848.
Gusman, E., Leone, C., Helmerhorst, E., Nunn, M., Flora, B., Troxler, R., Oppenheim, F.G. (2004):Arch. Oral. Biol.,49, 11–12.
Hägewald, S.J., Fishel, D.L.W., Christan, C.E.B., Bernimoulin, J.P., Kage, A. (2003):Eur. J. Oral Sci.,111, 203–208.
Henskens, Y.M.C., Van Der Weijden, F.A., Van Den Keijbus, P.A.M., Veerman, E.C.I. (1996):J. Periodontol.,67, 205–212.
Kaufman, E., Lamster, I.B. (2002):Crit. Rev. Oral Biol. Med.,13, 197–212.
Kishimoto, E., Hay, D.I., Kent, R. (1990):J. Dent. Res.,69, 1741–1745.
Levine, M.J. (1993): Malamud D, Tabak L, editors.Ann. NY. Acad. Sci.,694, 11–16.
López-Solís, R.O., Kemmerling, U., Ramos, A., Hoecker, G. (2003a):J. Cell. Biochem.,90, 945–956.
López-Solis, R.O., Puente, M., Morales-Bozo, I., Kemmerling U, Diaz F. (2003b):Biochim. Biophys. Acta,1621, 41–47.
López-Solis, R.O., Miranda, D. (1986):Biochim. Biophys. Acta,881, 422–429.
Laemmli, U.K. (1970):Nature,227, 680–685.
Lundy, F.T., Al-Hashimi, I., Rees, T.D., Lamey, P.J. (1997):Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod.,83, 252–258.
Morales-Bozo, I., Domínguez, P., López-Solís, R.O. (1998):Rev. Med. Chile,126, 538–547.
Nieuw Amerongen, A.V., Strooker, H., Oderkerk, C.H., Bank, R.A., Henskens, Y.M.C., Schenkels, L.C.P.M.,et al. (1992):J. Oral Pathol. Med.,21, 203–208.
Rudney, J.D. (1989):Arch. Oral Biol.,34, 499–506.
Schwartz, S.S., Zhu, W.X., Sreebny, L.M. (1995):Arch. Oral Biol.,40, 949–958.
Sonesson, M., Eliasson, L., Matsson, L. (2003):Arch. Oral Biol.,48, 535–539.
Sreebny, L.M., Zhu, W.X. (1996):Adv. Dent. Res.,10, 17–24.
Streckfus, C.F., Bigler, L.R. (2002):Oral Dis.,8, 69–76.
Veerman, E.C.I., Van den Keybus, P.A.M., Vissink, A., Nieuw Amerongen, A.V. (1996):Eur. J. Oral. Sci.,104, 346–352.
Vitorino, R., Lobo, M.J.C., Ferrer-Correira, A.J., Dubin, J.R., Tomer, K.B., Dominguez, P.M.,et al. (2004):Proteomics,4, 1109–1115.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Morales-Bozo, I., Urzúa-Orellana, B., Domínguez, P. et al. Patterns and variability in electrophoretic polypeptide profiles of human saliva in a healthy population. J. Physiol. Biochem. 62, 179–188 (2006). https://doi.org/10.1007/BF03168467
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03168467