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13C−1H heteronuclear dipolar correlation studies of the hydrogen bonding of the quinones inRhodobacter sphaeroides R26 reaction centers

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Abstract

The photosynthetic reaction center (RC) of the photosynthetic bacteriumRhodobacter sphaeroides R26 contains two quinones, QA and QB. Solid-state heteronuclear (1H−13C) dipolar correlation spectroscopy has been used to study the binding of the quinones in the ground state for RCs reconstituted with l-13C ubiquinone-10. Lee-Goldburg cross-polarization buildup curves are recorded to determine distancesr CH between the l-13C carbon labels and the protons involved in the polarization transfer. The l-13C of both QA and QB have intermolecular correlations with protons that resonate downfield, in the region of hydrogen-bonding protons. The distances between the carbon labels and the correlated protons are short, 0.21±0.01 nm. Hence the nuclear magnetic resonance provides evidence for strong hydrogen-bonding interactions at the l-C=O of both QA and QB for RCs in the ground state. The environment of the l-13C of the QB is structurally heterogeneous compared to that of the QA. The data can be reconciled with a strong H-bonding interaction of the l-C=O of QA with Ala M260 NH, and with complex hydrogen bonding involving NH of Ile-L224 and of Gly-L225, and possibly the Ser-L223 hydroxyl group of the l-C=O of the QB, in the proximal site.

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Authors and Affiliations

  1. Gorlaeus Laboratories, Leiden Institute of Chemistry, Leiden University, POB 9502, 2300 RA, Leiden, The Netherlands

    B. J. van Rossum, W. B. S. van Liemt, J. Lugtenburg & H. J. M. de Groot

  2. Huygens Laboratories, Leiden Institute of Physics, Leiden University, Leiden, The Netherlands

    P. Gast

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  1. B. J. van Rossum
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  2. W. B. S. van Liemt
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  3. P. Gast
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  4. J. Lugtenburg
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  5. H. J. M. de Groot
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van Rossum, B.J., van Liemt, W.B.S., Gast, P. et al. 13C−1H heteronuclear dipolar correlation studies of the hydrogen bonding of the quinones inRhodobacter sphaeroides R26 reaction centers. Appl. Magn. Reson. 31, 145–158 (2007). https://doi.org/10.1007/BF03166252

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  • DOI: https://doi.org/10.1007/BF03166252

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