Abstract
In this work, we investigated the interaction of a number of novel pH-sensitive spin probes (imidazoline- and imidazolidine-based nitroxides) with isolated chloroplasts. The light-induced changes in the electron paramagnetic resonance signals of these radicals are determined by several factors: ΔpH-driven accumulation of nitroxides inside the thylakoids, consumption (or evolution) of oxygen by chloroplasts, and irreversible loss of spin probe paramagnetism. A comparative study of these radicals characterized by different pKa allowed us to conclude that these spin probes can be used as pH indicators for monitoring the intrathylakoid pH- and light-induced changes in oxygen concentration in chloroplast suspension.
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Ptushenko, V.V., Ikryannikova, L.N., Grigor’ev, I.A. et al. Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplasts. Appl. Magn. Reson. 30, 329–343 (2006). https://doi.org/10.1007/BF03166204
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DOI: https://doi.org/10.1007/BF03166204