Abstract
It is stressed that the presence of a metal ion directly bound to a protein can introduce elements of complexity, such as vibronic coupling, which cannot be ruled outa priori. As a typical example, two copper proteins, azurin and plastocyanin, have been considered. Electron paramagnetic resonance spectra of the two proteins, recorded in the range 4–200 K and with different cooling rates, support the hypothesis of a prominent role played by vibronic interactions as well as by the conformational substate distribution.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Frauenfelder, A. in: Amorphous and Liquid Materials (E. Luscheret al. eds.) pp 3–18 and references therein, Martinus Nijhoff Publ.: Dordrecht 1987.
Goldanskii, V.I., Krupyanskii, Y.F.: Quart. Rev. Biophys.22, 39 (1989)
Brill, A.S. in: Tunnelling in Biological Systems (B. Chanceet al. eds.) pp 561–568, Academic Press.: New York 1979.
Bacci, M.: Chem. Phys.88, 39 (1984)
Elber, R., Karplus, M.: Science235, 318 (1987)
Singh, G.P., Schink, H.J., Loneysen, H.W., Parak, F., Hunklinger, S.: Z. Phys.B55, 23 (1984)
Doster, W., Bachleitner, A., Duman, R., Hielb, M., Luscher, E.: Biophys. J.50, 213 (1986)
Devault, D.: Quart. Rev. Biophys.13, 387 (1980)
Stein, D.L.: Proc. Natl. Acad. Sci.82, 3670 (1985)
Yang, I.S., Anderson, A.C.: Phys. Rev.B34, 2942 (1986); Phys. Rev.B35, 9305 (1987)
Kohler, W., Friedrich, J., Scheer, H.: Phys. Rev.A37, 660 (1988)
Iben, E.T., Braunstein, D., Doster, W., Frauenfelder, H., Hong, M.K., Johnson, J.B., Luck, S., Ormos, P., Schulte, A., Steinbach, P.J., Xie, A.H., Young, R.D.: Phys. Rev. Lett.62, 1916 (1989)
Phillips, W.A. (ed.) Amorphous Solids: Low Temperature Properties, vol. 24., Springer: Berlin 1981.
Cannistraro, S., Giugliarelli, G., Marzola, P., Sacchetti, F.: Solid State Commun.68, 369 (1988)
Cannistraro, S.: J. Phys. (France)51, 59 (1990)
Bacci, M.: Structure and Bonding55, 67 (1983)
Bacci, M.: Chem. Phys.104, 191 (1986); Chem. Phys. Lett.134, 143 (1987); Japan J. Appl. Phys.27, L1699 (1988)
Wang, C.X., Giugliarelli, G., Cannistraro, S., Fini, C.: Il Nuovo CimentoD8, 76 (1986)
Giugliarelli, G., Cannistraro, S.: Il Nuovo CimentoD4, 194 (1984)
Giugliarelli, G., Cannistraro, S.: Chem. Phys.98, 115 (1985)
Giugliarelli, G., Tancini, P., Cannistraro, S.: J. Phys. E: Sci. Instrum.22, 702 (1989)
Brill, A.S.: Transition Metals in Biochemistry, Springer-Verlag: New York 1977.
Bacci, M., Cannistraro, S.: Chem. Phys. Lett.133, 109 (1987)
Bacci, M., Cannistraro, S.: J. Chem. Soc. Farad. Trans. I83, 3693 (1987)
O’Brien, M.C.M.: Proc. R. Soc. LondonA281, 323 (1964)
Ham, F.S. in: Electron Paramagnetic Resonance, p. 1 (Geschwind, S. ed.) Plenum: New York 1972.
Riley, M.J., Hitchman, M.A., Reinen, D.: Chem. Phys.102, 11 (1986)
Bersuker, I.B., Polinger, V.Z.: Vibronic Interaction in Molecules and Crystals, Springer-Verlag: Berlin 1989.
Bill, H. in: The Dynamical Jahn-Teller Effect in Localized System, p. 709 (Yu.E. Perlin and L. Wagner eds.) North-Holland: Amsterdam 1984.
Guss, J.M., Freeman, H.C.: J. Mol. Biol.169, 521 (1983)
Norris, G.E., Anderson, B.F., Baker, E.N.: J. Mol. Biol.165, 501 (1983)
Duval, F, Champagnon, B., Lacroix, R.: J. Phys. (Paris)37, 1391 (1976)
Silver, B.L., Getz, D.: J. Chem. Phys.61, 638 (1974)
Misra, S.K., Wang, C.: Phys. Rev.B41, 1 (1990)
Bacci, M.: Chem. Phys.40, 237 (1979)
Hagen, W.R.: J. Magn. Res.44, 447 (1981)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bacci, M., Cannistraro, S. Role of vibronic coupling and of conformational substate distribution in determining the features of copper-protein EPR spectra. Appl Magn Reson 1, 369–378 (1990). https://doi.org/10.1007/BF03166020
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03166020