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99mTc labeling white blood cells with a simple technique: Clinical application

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Abstract

Several radionuclides and different methods have been employed as cellular labels to study inflammatory sites in man. Here we present the results obtained with white blood cells (WBC) labeled with99mTc using a simple and low cost new technique (SnTec). WBC were incubated with 12 μg/ml of stannous chloride for 10 min at room temperature. Then99mTc was added. After 10 min, the99mTc-labeled WBC were washed and injected into the patient. Comparison studies with99mTclabeled WBC using the HMPAO technique were carried out in patients with suspected osteomyelitis. Since the results are similar with both methods, we suggest the use of SnTec to label WBC, in cases of inflammatory diseases.

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Authors and Affiliations

  1. Departamento de Biofísica e Biometria, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Av. 28 de Setembro, 87, CEP 20551-030, Rio de Janeiro-RJ-Brasil

    Bianca Gutfilen & Mario Bernardo-Filho

  2. Serviço de Medicina Nuclear, Hospital Naval Marcilio Dias., RJ

    Marcos Pinto Pellini, Jacqueline de Roure e Neder, José Luiz Medeiros de Amarante, Maria Garděnia Evangelista & Sérgio Roberto Fernandes

  3. Pesquisa Básica, Instituto Nacional de Căncer, RJ

    Mario Bernardo-Filho

Authors
  1. Bianca Gutfilen
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  2. Marcos Pinto Pellini
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  3. Jacqueline de Roure e Neder
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  4. José Luiz Medeiros de Amarante
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  5. Maria Garděnia Evangelista
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  6. Sérgio Roberto Fernandes
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  7. Mario Bernardo-Filho
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Gutfilen, B., Pellini, M.P., de Roure e Neder, J. et al. 99mTc labeling white blood cells with a simple technique: Clinical application. Ann Nucl Med 8, 85–89 (1994). https://doi.org/10.1007/BF03164991

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  • DOI: https://doi.org/10.1007/BF03164991

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