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Radioimmunoscintigraphy of colorectal cancer with technetium-99m-labeled murine anti-carcinoembryonic antigen monoclonal antibody in athymic nude mice

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Abstract

Technetium-99m (Tc-99m) is an ideal radionuclide for clinical use. A murine monoclonal antibody (Mab) designated F33-104 binds to specific parts of carcinoembryonic antigen (CEA). In the present study, intact Mab F33-104 was labeled with Tc-99m, and the immunoreactivity and biodistribution of Tc-99m-labeled F33-104 were studied in athymic nude mice bearing human colorectal cancer xenografts. Mab F33-104, reduced under optimal conditions, was quickly and stably tagged with Tc-99m without loss of immunoreactivity. Higher tumor uptake of Tc-99m-labeled F33-104 was noted in the biodistribution, resulting in a higher localization index and specific-to-non-specific tumor ratio than those of radioiodinated F33-104. These results suggest the potential of Tc-99m-labeled Mab F33-104 for the radioimmunoimaging of colorectal cancer.

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Abbreviations

CEA:

carcinoembryonic antigen

Tc-99m:

technetium-99m

I-125:

iodine-125

Mab:

monoclonal antibody

2-ME:

2-mercaptoethanol

HMDP:

hydroxyl methylene diphosphonate

In-111:

indium-111

PBS:

phosphate buffered saline

hCG:

human chorionic gonadotropin

RIA:

radioimmunoassay

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Authors and Affiliations

  1. Department of Nuclear Medicine, Gunma University, School of Medicine, 3-39-22 Showa-machi, Maebashi-shi, 371, Gunma, Japan

    Naoyuki Watanabe & Noboru Oriuchi

  2. Department of Radiology, Takasaki National Hospital, Japan

    Sumio Sugiyama

  3. First Department of Biochemistry, School of Medicine, Fukuoka University, Japan

    Masahide Kuroki & Yuji Matsuoka

Authors
  1. Naoyuki Watanabe
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  2. Noboru Oriuchi
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  3. Sumio Sugiyama
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  4. Masahide Kuroki
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  5. Yuji Matsuoka
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Watanabe, N., Oriuchi, N., Sugiyama, S. et al. Radioimmunoscintigraphy of colorectal cancer with technetium-99m-labeled murine anti-carcinoembryonic antigen monoclonal antibody in athymic nude mice. Ann Nucl Med 8, 23–30 (1994). https://doi.org/10.1007/BF03164983

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  • DOI: https://doi.org/10.1007/BF03164983

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