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Changes in CA125 release and surface expression caused by drugs in uterine cervix adenocarcinoma cells

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Abstract

The effect of drugs on the release of CA125 antigen and the binding of anti-CA125 mono-clonal antibody (MoAb) to malignant cells was evaluatedin vitro. TMCC-1, uterine cervical adenocarcinoma cells, were exposed to dexamethasone (DEX), sodiumn-butyrate (NaB), dibutyryl cyclic AMP (dbcAMP), retinoic acid (RA), calcitriol (VD3), and interferon-γ (IFN-γ). NaB, RA and VD3 increased CA125 release per cell and125I-labeled anti-CA125 MoAb binding to the cells. DEX also increased the125I-labeled anti-CA125 MoAb binding to the cells, and CA125 antigen release per cell was also slightly increased. IFN-γ suppressed both CA125 release and125I-labeled MoAb binding. A combination of DEX, VD3 and RA and increased the binding of MoAb to TMCC-1 cells, but the amount of bound MoAb was not significantly different from that obtained by single drug treatment. DbcAMP had no significant effect on enhancing MoAb binding. Drugs can increase the binding of anti-CA125 MoAb to malignant cells and they may be applied to increase the tumor uptake of radiolabeled MoAbsin vivo.

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Authors and Affiliations

  1. Department of Nuclear Medicine, Kyoto University School of Medicine, 54 Shogoin Kawaharacho, Sakyo-ku, 606-01, Kyoto, Japan

    Toshiharu Nakai, Harumi Sakahara, Makoto Shirato, Hisataka Kobayashi, Makoto Hosono, Tsuneo Saga & Junji Konishi

  2. Department of Nuclear Medicine, Gunma University School of Medicine, Maebashi

    Keigo Endo

  3. Department of Gynecology and Obstetrics, Tokyo Medical College, Tokyo

    Masaru Sakamoto

Authors
  1. Toshiharu Nakai
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  2. Harumi Sakahara
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  3. Keigo Endo
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  4. Makoto Shirato
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  5. Hisataka Kobayashi
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  6. Makoto Hosono
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  7. Tsuneo Saga
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  8. Masaru Sakamoto
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  9. Junji Konishi
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Nakai, T., Sakahara, H., Endo, K. et al. Changes in CA125 release and surface expression caused by drugs in uterine cervix adenocarcinoma cells. Ann Nucl Med 7, 133–139 (1993). https://doi.org/10.1007/BF03164956

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  • DOI: https://doi.org/10.1007/BF03164956

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