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Temperature dependence of theg=6 EPR linewidth in high spin FeIII myoglobin samples

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Abstract

The temperature dependence of theg=6 line of high spin FeIII aqueous mixed water-glycerol myoglobin samples has been investigated by EPR spectroscopy. The trend with the temperature of the linewidth has been analyzed by taking into account both a temperature-independent contribution from the frozen structural heterogeneity (conformational substate distribution) and the relaxation processes. The temperature-dependent line broadening process due to the spin-lattice relaxation has been singled out by simulation of the experimental data and put into relationship to the density of the vibrational states. The effect on the vibrational modes of the protein-solvent system, as induced by the addition of large amount of the glass-making solvent, glycerol, is discussed.

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Authors and Affiliations

  1. Dipartimento di Scienze Ambientali, Sezione Chimica e Fisica, Universita’ della Tuscia, Viterbo, Italy

    S. Cannistraro

  2. Dipartimento di Fisica dell’Universita’, Unita’ INFM-CNR, I-06100, Perugia, Italy

    A. R. Bizzarri & S. Cannistraro

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  1. A. R. Bizzarri
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  2. S. Cannistraro
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Bizzarri, A.R., Cannistraro, S. Temperature dependence of theg=6 EPR linewidth in high spin FeIII myoglobin samples. Appl. Magn. Reson. 6, 575–586 (1994). https://doi.org/10.1007/BF03162646

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  • DOI: https://doi.org/10.1007/BF03162646

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