Abstract
Proton NMR spectra of perchloric acid extracts of methyl cholantherene induced tumors grown in rats have been analyzed and compared with the normal and the treated tumor tissue samples. Well-resolved resonances from numerous low-molecular weight compounds including various amino acids, nucleotides, choline, creatine, phosphocreatine etc. were observed and assigned using pH titration, 2D NMR and by comparison with the spectra of model compounds. Significant differences were noticed in the spectra of the tumor and the normal tissue samples. Ratios of metabolite levels were calculated for the normal, tumor and treated tumor tissues which are shown as good markers to assess the state of the tumor and their response to treatment.
Similar content being viewed by others
References
Gadian D.G.: Nuclear Magnetic Resonance and its Application to Living Systems. Oxford: Clarendon Press 1982.
Rothman D.L., Behar K.L., Hetherington H.P., Shulman R.G.: Proc. Natl. Acad. Sci. (USA)81, 6330–6334 (1984)
Shulman R.G.: TIBS13, 37–39 (1988)
Bottomley P.A.: Radiology170, 1–15 (1989)
Narayana P.A., Jackson E.F.: Curr. Sci.61, 340–351 (1991)
Evanochko W.T., Sakai T.T., Ng T.C., Ramakrishna N., Kim H.D., Zeidler R.B., Ghanta U.K., Brockman R.W., Schiffer L.M., Braunschweiger P.G., Glickson J.D.: Biophys. Acta805, 104–116 (1984)
Fan T.W.M., Higashi R.M., Lane A.N., Jardetzky O.: Biochem. Biophys. Acta882, 154–167 (1986)
Komoroski R.A., Fowler A.H., Lubansky H.J., Rice C.D., Jay W.M.: Spectrosc. Lett.24, 1123–1144 (1991)
Fowler A.H., Pappas A.A., Holder J.C., Finkbeiner A.E., Dalrymple G.V., Mullins M.S., Sprigg J.R., Komoroski R.A.: Magn. Reson. Med.25, 140–147 (1992)
Nagarajan B., Sankaran S.: Ind. J. Cancer10, 83–84 (1973)
Munch Peterson B., Trysted G., Dupont B.: Exp. Cell Res.79, 249–256 (1973)
Sendhil Velan S., Jagannathan N.R., Saravanan K., Nagarajan B.: Med. Sci. Res.19, 609–611 (1991)
Nagarajan V.: M. Sc. Dissertation, University of Madras 1993.
Sherry A.D., Malloy C.R., Jeffrey F.M.H., Chavez F., Sreve H.K.: J. Magn. Reson.89, 391–398 (1990)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jagannathan, N.R., Velan, S.S. Therapeutic response of tumors by in-vitro proton nuclear magnetic resonance spectroscopy. Appl. Magn. Reson. 5, 357–367 (1993). https://doi.org/10.1007/BF03162532
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03162532