Abstract
There are many advantages to carrying out electron paramagnetic resonance experiments at very high frequencies, either as part of a multifrequency strategy for solving problems or for special characteristics of high frequencies. These special characteristics include the potential for high point sensitivity, enhanced resolution, separation of similar species, altered sensitivity to motion, suppression of motional effects, and many others. This paper describes a three-millimeter-wavelength (W-band, 94 GHz) EPR spectrometer built for a multi-user facility and illustrates with some examples, most of them being disordered systems. One significant example is the oxidized primary reaction center, P +865 , isolated from the red photosynthetic bacteriaRhodobacter Sphaeroides R-26. The W-band technique applied to both centers isolated from bacteria grown from either deuterated or ordinary growth media allows extraction of the fullg anisotropy in these centers and sets the stage for multifrequency EPR spectroscopy to yield a full analysis of the various contributions to linewidths in these systems.
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Adapted in substantial part from the PhD Dissertation of Wei Wang, University of Illinois, Urbana, April 1993.
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Wang, W., Belford, R.L., Clarkson, R.B. et al. Very high frequency EPR — 94 GHz instrument and applications to primary reaction centers from photosynthetic red bacteria and to other disordered systems. Appl. Magn. Reson. 6, 195–215 (1994). https://doi.org/10.1007/BF03162490
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DOI: https://doi.org/10.1007/BF03162490