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High-frequency EPR studies on cofactor radicals in photosystem I

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Abstract

Electron paramagnetic resonance (EPR) spectroscopy at W-band (94 GHz) is used to resolve theg-tensors of the radical ions of the primary chlorophyll donor P +⋅700 and the quinone acceptor A −⋅1 in photosystem I. The obtainedg-tensor principal values are compared with those of the isolated pigment radicals in organic solvents and the shifts are related to an impact of the protein environment. P +⋅700 has been investigated in photosystem I single crystals at 94 GHz. W-band EPR applied to the photoinduced radical pair P +⋅700 A −⋅1 is used to correctly assign theg-tensor axes of P +⋅700 to the molecular structure of the primary donor. Density functional theory calculations on a model of A −⋅1 in its binding pocket derived from the recent crystal structure of photosystem I were utilized to correlate experimental magnetic resonance parameters with structural elements of the protein.

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Authors and Affiliations

  1. Max-Volmer-Laboratorium, Technische Universität Berlin, Berlin, Germany

    C. Teutloff, W. Hofbauer, M. Stein, R. Bittl & W. Lubitz

  2. Institut für Experimentalphysik, Freie Universität Berlin, Berlin, Germany

    S. G. Zech & R. Bittl

  3. Max-Planck-Institut für Strahlenchemie, Mülheim/Ruhr, Germany

    W. Lubitz

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  1. C. Teutloff
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  2. W. Hofbauer
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  3. S. G. Zech
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  4. M. Stein
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  5. R. Bittl
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  6. W. Lubitz
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Teutloff, C., Hofbauer, W., Zech, S.G. et al. High-frequency EPR studies on cofactor radicals in photosystem I. Appl. Magn. Reson. 21, 363–379 (2001). https://doi.org/10.1007/BF03162414

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  • DOI: https://doi.org/10.1007/BF03162414

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