Abstract
In order to understand the role of monoamines in cerebral ischemia, 3-methyoxy-4-hydroxyphenylglycol(MHPG), 5-hydroxyindoleacetic acid (5-HIAA), and homovanillic acid(HVA), the three major unconjugated monoamine metabolites in cerebrospinal fluid (CSF), of 33 patients and 18 controls were measured with high performance liquid chromatography. Results showed all three metabolites were raised in patients with severe ischemia, but only MHPG and 5-HIAA were elevated significantly, MHPG changes more quickly and regularly as a consequence of cerebral ischemia than the two others. A positive correlation between any pair of metabolites was found in controls and in patients in the first week after stroke, but not at the end of the second week. Computer assisted multivariate analysis indicated 5-HIAA and MHPG correlated more closely with the state of illness in the acute stage, whereas HVA correlated the least. Possible explanations for the changes of CSF levels of amine metabolites are discussed.
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Bell W. H., III, Sundt T. M., and Nofzinger J. D. (1967) The response of cortical vessels to serotonin in experimental cerebral infarction.J. Neurosurg. 26, 203–212.
Bralet J., Beley P., Bralet A. M., and Beley A. (1979) Catecholamine levels and turnover during brain ischemia in the gerbil brain.Stroke 10, 165–167.
Gaudet R., Welch K. M. A., Chabi E., and Wang T. P. (1978) Effect of transient ischemia on monoamine levels in the cerebral cortex of gerbils.J. Neurochem. 30, 751–757.
Gershon M. D. (1977) Cellular Biology of Neurons, part 1,Handbook of Physiology—The Nervous System, vol. 1 (Kandel E. R., ed.), pp. 579–584, American Physiological Society, Bethesda, MD.
Harrison M. J. G., Marsden C. D., and Jenner P. (1979) Effect of experimental ischemia on neurotransmitter amines in the gerbil brain.Stroke 10(2), 165–167.
Hornig C. R., Dorndorf W., and Agnoli A. L. (1986) Hemorrhagic cerebral infarction—A prospective study.Stroke 17, 179–185.
Lade C. R., Gullner H. G., Ploinsky R. J., Ebert M. H., Ziegler M. H., and Bartter F. C. (1981) Essential hypertension: Central and peripheral norepinephrine.Science 221, 955–957.
Meyer J. S., Welch K. M. A., Okamoto S., and Shimazu K. (1974) Disordered neurotransmitter function. Demonstration by measurement of norepinephrine and 5-HT in CSF of patients with recent cerebral infarction.Brain 97, 655–664.
Osterholm J. L. and Bell J. (1969) Experimental effects of free serotonin on the brain and its relation to brain injury. Part 1: the neurological consequences of intracerebral serotonin injection.J. Neurosurg. 31, 408–412.
Scheinin M., Chang W. H., Kipk K. L., and Linnoila M. (1983) Simultaneous determination of MHPG, 5-HIAA, and HVA in CSF with HPLC using electrochemical detection.Anal. Biochem. 131, 246–253.
Welch K. M. A., Chabi E., Euckingham J., Bergin B., Achar V. S., and Meyer J. S. (1977) Catecholamine and 5-HT levels in ischemic brain. Influence ofp-chrophenylalamine.Stroke 8, 341–346.
Welch K. M. A., Hashi K., and Meyer J. S. (1973) Cerebrovascular response to intracarotid injection of serotonin before and after middle cerebral artery occlusion.J. Neurol. Neurosurg. Psychiat. 36, 721–735.
White R. P., Hagen A., and Robertson J. T. (1976) Experimental evaluation of the spasmogenicity of dopamine on the basilar artery.J. Neurosurg. 44, 45–49.
Wilkinson L. (1984) CORR and MGLH,SYSTAT, pp. 103–180, SYSTAT Inc., Evanston, IL.
Wood J. H. (1980) Site of origin and cerebrospinal fluid concentration gradients,Neurobiology of Cerebrospinal Fluid, vol. 1 (Wood J. H., ed.), pp 55–58, Plenum, New York, NY.
Wurtman R. J. and Zervas N. T. (1974) Monoamine neurotransmitters and the pathophysiology of stroke and central nervous system trauma.J. Neurosurg. 40, 34–36.
Yashon D., Blocker D., Brown R. J., and Hunt W. E. (1975) Quantitative in vitro vasoactivity of 5-hydroxytryptamine on the human basilar artery.Surg. Neurol. 3, 295–300.
Young S. N., Gauthier S., Anderson G. M., and Purdy W. C. (1980) Tryptophan, 5-HIAA and indoleacetic acid in human CSF: interrelationships and the influence of age, sex, epilepsy and anticonvulsant drugs.J. Neurol. Neurosurg. Psychiat. 43, 438–445.
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Tang, H.Z., Lu, X.F., Wang, K.J. et al. Monoamine metabolites in cerebrospinal fluid during and after acute cerebral ischemia. Molecular and Chemical Neuropathology 10, 77–85 (1989). https://doi.org/10.1007/BF03159715
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DOI: https://doi.org/10.1007/BF03159715