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Macrofagi e risposta immunitaria. I. effetti del blocco dei macrofagi peritoneali sulla risposta immunitaria primaria in topi BALB/c. studio istologico ed immunologico in funzione del tempo di somministrazione dell’agente bloccante

Macropages and immune responses. I. Time-dependent effect of peritoneal macrophages blockade on primary immune responses in BALB/c mice. Histological and immunological study

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La Ricerca in Clinica e in Laboratorio

Riassunto

Nel corso del presente esperimento abbiamo studiato gli effetti di un’unica somministrazione intraperitoneale di particelle di carbone colloidale, vale a dire di un agente ingolfante i macrofagi, sul quadro istologico e sulla reattività immunitaria primaria ai G.R.M., nel topo di ceppo BALB/c. Il carbone colloidale è stato iniettato a diversi intervalli di tempo prima, contemporaneamente o dopo l’immunizzazione. I risultati indicano che il trattamento con carbone deprime la risposta immunitaria primaria solo se questo è stato somministrato a brevi intervalli prima dell’antigene. Di conseguenza i macrofagi sembrano giocare un ruolo molto importante negli eventi cellulari associati alla risposta immunitaria primaria ai G.R.M. Appare anche evidente dalle riportate osservazioni che l’interazione macrofago-antigene avviene effettivamente e precede l’interazione fra T-linfocita ed antigene. Inoltre si ritiene di poter suggerire che i macrofagi peritoneali costituiscano un terzo tipo cellulare necessario per l’instaurarsi della risposta immunitaria primaria ad alcuni antigeni particolati somministrati per via peritoneale.

Summary

The effects of a single i.p. administration of colloidal carbon particles, i.e. of a macrophage ingulfing agent, have been studied on histology and primary immune response to SRBC in BALB/c mice. Colloidal carbon was given at different times before, with or after sheep erythrocytes. The results indicate that, under the experimental conditions tested, carbon treatment depresses primary immune response only if given shortly before antigenic stimulation. Accordingly, macrophages may play a role on early cellular events associated with the primary immune response to SRBC. It appears clear from these observations that macrophage-antigen interaction takes place and antecedes T-lymphocyte-antigen interaction. Furthermore our study indicates that peritoneal macrophages might be considered as a third cell type necessary to initiate the primary immune response to some particulate antigens.

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Authors and Affiliations

  1. Istituto di Anatomia ed Istologia Patologica II dell’Università di Roma viale Regina Elena, 324 00161, Roma, Italy

    Carlo D. Baroni, Ugo Di Tondo, Paolo C. Pesando, Luigi G. Spagnoli & Carlo D. Baroni

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  1. Carlo D. Baroni
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  2. Ugo Di Tondo
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  3. Paolo C. Pesando
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  4. Luigi G. Spagnoli
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  5. Carlo D. Baroni
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Investigation supported by a grant from the National Research Council of Italy (CNR).

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Baroni, C.D., Di Tondo, U., Pesando, P.C. et al. Macrofagi e risposta immunitaria. I. effetti del blocco dei macrofagi peritoneali sulla risposta immunitaria primaria in topi BALB/c. studio istologico ed immunologico in funzione del tempo di somministrazione dell’agente bloccante. La Ricerca Clin. Lab. 1, 110–132 (1971). https://doi.org/10.1007/BF03054455

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