Skip to main content
SpringerLink
Log in

Growth and metabolism in cotton and tobacco callus cultures

  • Published:
Proceedings / Indian Academy of Sciences

Abstract

Activities of key enzymes of glycolytic and pentose phosphate pathways and krebs cycle were less in tobacco callus than in cotton callus cultures grown under identical conditions. The initial period of growth was characterized by high activities of invertase, acid phosphatase, hexokinase, fructose diphosphate aldolase, malate dehydrogenase, succinate dehydrogenase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase; the activities of these enzymes declined during the remainder period of growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akemine T, Kikuta Y and Tagawa T 1975 Effect of kinetin and naphthaleneacetic acid application on the respiratory metabolism during callus development in potato tuber tissue culturedin vitro;J. Fac. Agric. Hokkaido Univ. 58 247–261

    Google Scholar 

  • Bacon J S D 1955 Methods for measuring transglycosylase activity of invertases;Methods Enzymol. 1 149–158

    Article  Google Scholar 

  • Bernfeld P 1955 Amylases, α and β;Methods Enzymol. 1 149–158

    Article  CAS  Google Scholar 

  • de Jong D W 1967 An investigation of the role of plant peroxidase in cell wall development by the histochemical method;Cytochemistry 15 335–346

    Google Scholar 

  • Filner B and Klein A O 1968 Changes in enzymatic activities in etiolated bean seedling leaves after a brief illumination;Plant Physiol. 43 1587–1596

    Article  PubMed  CAS  Google Scholar 

  • Fowler M W 1971 Studies on the growth in culture of plant cells. XIV. Carbohydrate oxidation during the growth ofAcer pseudoplatanus L. cells in suspension culture;J. Exp. Bot. 22 715–724

    Article  CAS  Google Scholar 

  • Givan C V and Collin H A 1967 Studies on the growth in culture of plant cells. II. Changes in respiration rate and nitrogen content associated with the growth ofAcer pseudoplatanus L. cells in suspension culture;J. Exp. Bot. 18 321–331

    Article  Google Scholar 

  • Hassid W Z and Neufeld E F 1964 Quantitative determination of starch in plant tissues;Methods. Carbohydr. Chem. 4 33–36

    CAS  Google Scholar 

  • Jagannathan V, Kartar Singh and Damodaran V 1956 Carbohydrate metabolism in citric acid fermentation. Purification and properties of aldolase fromAspergillus niger;Biochem. J. 63 94–105

    PubMed  CAS  Google Scholar 

  • Joshi M D and Jagannathan V 1966 Hexokinase;Methods Enzymol. 2 371–375

    Article  Google Scholar 

  • Kavi Kishor P B and Mehta A R 1983 Osmotic involvement and organogenesis in callus cultures ofNicotiana tabacum L;Proc. Indian Natl. Sci. Acad. B49 729–734

    Google Scholar 

  • Kavi Kishor P B and Mehta A R 1986 The influence of applied carbohydrates on the growth and amylase activity in cultured cotton cells;Indian Bot. Rep. 5 19–23

    CAS  Google Scholar 

  • Kavi Kishor P B and Mehta A R 1987 Effects of different sugars on invertase activity in cotton callus cultures;J. Rec. Adv. Appl. Sci. 2 244–253

    Google Scholar 

  • Komamine A, Morigaki T and Fujimura T 1978 Metabolism in synchronous growth and differentiation in plant tissue and cell cultures; inFrontiers of plant tissue culture (ed.) T A Thorpe (University of Calgary) pp 159–168

  • Komamine A and Shimizu T 1975 Changes in some enzyme activities and respiration in the early stage of callus formation in a carrot root tissue culture;Physiol. Plant. 33 47–52

    Article  CAS  Google Scholar 

  • Lowry O H, Rosebrough N J, Farr A L and Randall R J 1951 Protein measurement with the Folin phenol reagent;J. Biol. Chem. 193 265–275

    PubMed  CAS  Google Scholar 

  • Malik C P and Singh M B (eds) 1980 Plant enzyme assays—some examples; inPlant enzymology and histoenzymology (New Delhi: Kalyani Publishers) pp 47–84

    Google Scholar 

  • Nash D T and Boll W G 1975 Carbohydrate nutrition of Paul’s Scarlet Rose cell suspensions;Can. J. Bot. 53 179–185

    Article  CAS  Google Scholar 

  • Nash D T and Davies M E 1972 Some aspects of growth and metabolism of Paul’s Scarlet Rose cell suspensions;J. Exp. Bot. 23 75–91

    Article  CAS  Google Scholar 

  • Nelson N 1944 A photometric adaptation of the Somogyi method for the determination of glucose;J. Biol. Chem. 153 275–279

    Google Scholar 

  • Ochoa S 1955 Malic dehydrogenase from pig heart;Methods Enzymol. 1 735–739

    Article  CAS  Google Scholar 

  • Palmer J M 1968 The effect of some plant growth substances on the induction of enzymatic activities in thin slices of plant tubers; inBiochemistry and physiology of plant growth substances (eds) F Wightman and G Setterfield (Ottawa: Runge Press) pp 401–415

    Google Scholar 

  • Pontremoli S and Grazi E 1966 6-Phosphogluconate dehydrogenase;Methods Enzymol. 9 137–141

    Article  CAS  Google Scholar 

  • Shimizu T, Clibton A, Komamine A and Fowler M W 1977 Changes in metabolite levels during growth ofAcer pseudoplatanus (sycamore) cells in batch suspension culture;Physiol. Plant. 40 125–129

    Article  CAS  Google Scholar 

  • Somogyi M 1952 Notes on sugar determination;J. Biol. Chem. 195 19–23

    CAS  Google Scholar 

  • Wilson S B 1971 Studies of the growth in culture of plant cells. XII. Properties of mitochondria isolated from batch cultures ofAcer pseudoplatanus cells;J. Exp. Bot. 22 725–734

    Article  CAS  Google Scholar 

  • Yem E W and Willis A J 1954 The estimation of carbohydrates in plant extracts by anthrone;Biochem. J. 57 508–514

    Google Scholar 

  • Zink M W and Veliky I A 1979 Acid phosphatases ofIpomoea sp. culturedin vitro. 1. Influence of pH and inorganic phosphate on the formation of phosphatases;Can. J. Bot. 57 739–753

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, M S University of Baroda, 390 002, Baroda, India

    A R Mehta

  2. Department of Botany, Kakatiya University, 506 009, Warangal, India

    P B Kavi Kishor

Authors
  1. P B Kavi Kishor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A R Mehta
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kavi Kishor, P.B., Mehta, A.R. Growth and metabolism in cotton and tobacco callus cultures. Proc. Indian Acad. Sci. 98, 277–282 (1988). https://doi.org/10.1007/BF03053799

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03053799

Keywords

Search

Navigation