Abstract
Penicillium pinophilum, Aspergillus quadricinctus andGliomastix murorum secreted a complete extra-cellular cellulase complex comprising of endo-β-glucanase, exo-β-glucanase and β-glucosidase. The enzyme production was optimum with absorbent cotton and a combination of ammonium sulphate, urea and peptone inPenicillium pinophilum, with carboxymethylcellulose-7 MT and ammonium chloride inAspergillus quadricinctus, and with absorbent cotton and peptone inGliomastix murorum. The biosynthesis of cellulase was repressed by sugars in the presence of cellulose and only negligible amounts of cellulolytic enzymes were produced when sugars formed the sole carbon sources.
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Bailey P J, Liese W, Roesch R, Keitich G and Afting E G 1969 Cellulase (β-1,4-glucan-4-glucanohydrolase) from the wood-degrading fungusPolyporus schweinitzii Fr. 1. Purification;Biochem. Biophys. Acta 185 381–391
Bastawde K B, Deshpande V V, Joglekar A V, Lakshmikantham B C, Chitra Mishra, Phansalkar S B, Mala Rao, Seeta R, Srinivasan M C and Jagannathan V 1977 Cellulolytic enzymes of aPenicillium strain;Proc. Bioconversion Symp., IIT, Delhi, pp 143–151
Berghem L E R and Pettersson L G 1973 The mechanism of enzymatic cellulose degradation. Purification of a cellulolytic enzyme fromTrichoderma viride active on highly ordered cellulose;Eur. J. Biochem. 37 21–30
Feniksova R V, Ulezlo I V and Shalamberidze N G 1970 The effect of various carbon sources on the formation of cellulolytic enzymes by the fungusTrichoderma sp. 185;Soobshch. Akad. Nauk. Gruz. SSR 57 689–692
Garg S K and Neelkantan S 1982 Effect of nutritional factors on cellulase enzyme and microbial protein production byAspergillus terreus and its evaluation;Biotechnol. Bioeng. 24 109–125
Gupta J K, Das N B and Gupta Y P 1972 Effect of cultural conditions on cellulase formation byTrichoderma viride;Agric. Biol. Chem. 36 1961–1967
Horton J C and Keen N T 1966 Regulation of induced cellulase synthesis inPyrenochaeta terrestris Gorenzet al by utilizable carbon compounds;Can. J. Microbiol. 12 209–220
Ismailova D Yu and Loginova L G 1975 Effect of some substances on the cellulase synthesis of the thermotolerant fungusAspergillus terreus 17 P;Prikl. Biokhim Mikrobiol. 11 676–681
Kassim E A 1982 Cellulase production by a strain ofMyrothecium sp.;J. Ferment. Technol. 60 381–383
Lizak Yu V 1975 Effect of different sources of nitrogen nutrition on formation of cellulolytic enzymes of dark-colored fungi;Mikrobiol. Zh. (Kiev) 37 693–699
Mandels M 1974Production and Application of Cellulase Laboratory Procedures. US Army Natick Development Center, Dec.
Mandels M and Reese E T 1957 Induction of cellulase inTrichoderma viride as influenced by carbon sources and metals;J. Bacteriol. 73 269–278
Miller G L 1959 Use of Dinitrosalicylic acid reagent for determination of reducing sugar;Anal. Chem. 31 426–428
Narania K and Reddy S M 1978 Cell wall degrading enzymes of five hyphomycetes. II. Influence of nitrogen on cellulases;Natl. Acad. Sci. Lett. 1 357–360
Rapp P, Knobloch U and Wagner F 1982 Repression of endo-1,4-β-glucanase formation inPenicillium janthinellum and product inhibition of its 1,4-β-glucanases and cellobiases;J. Bacteriol. 149 783–786
Reese E T and Mandels M 1963a Enzymatic hydrolysis of β-glucans; inAdvances in enzymatic hydrolysis of cellulose and related materials (ed) E T Reese (New York: Pergamon Press) pp 197–234
Reese E T and Mandels M 1963b Enzymic hydrolysis of cellulose and its derivatives;Methods Carbohydr. Chem. 3 139–143
Ryu D D Y and Mandels M 1980 Cellulases: biosynthesis and applications;Enzyme Microbiol. Technol. 2 91–102
Sadana J C, Lachke A H and Shewale J G 1979 Biochemistry of cellulose degradation and cellulose utilization for feeds and for protein;J. Sci. Ind. Res. 38 442–453
Shewale J G and Sadana J C 1978 Cellulase and β-glucosidase production by a basidiomycete species;Can. J. Microbiol. 24 1204–1216
Siu R G H and Sinden J W 1951 Effects of pH, temperature and mineral nutrition on cellulolytic fungi;Am. J. Bot. 38 284–290
Spano L, Medeiros J and Mandels M 1976 Enzymatic hydrolysis of cellulose wastes to glucose;Resource Recovery and Conservation 1 279–294
Sternberg D 1976 Production of cellulase byTrichoderma;Biotechnol. Bioeng. Symp. No.6 35–53
Sternberg D, Vijayakumar P and Reese E T 1977 β-Glucosidase: Microbial Production and effect on enzymatic hydrolysis of cellulose;Can. J. Microbiol. 23 139–147
Trivedi L S and Rao K K 1979 Production of Cellulolytic enzymes byAspergillus fumigatus;Indian J. Exp. Biol. 17 671–674
Wang D I C, Cooney C L, Wang S, Gordon J and Wang G Y 1978Proceedings 2nd Annual Symposium on Fuels from Biomass, Troy NY June 1978, II, p 537
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Sethi, J.K., Rawla, G.S. Cellulase production byPenicillium pinophilum, Aspergillus quadricinctus andGliomastix murorum . Proc. Indian Acad. Sci. 97, 63–73 (1987). https://doi.org/10.1007/BF03053341
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DOI: https://doi.org/10.1007/BF03053341