Abstract
The enzymes succinate dehydrogenase and peroxidase were localized histochemically in the leaf epidermal cells and in the young stems of:Annona squamosa L.,Coccinia indica W. and A.,Bougainvillea spectabilis Willd.,Azadirechta indica L.,Ficus benghalensis L.,Lantana camara L. var.aculeata, Mangifera indica L.,Manilkara hexandra (Roxb) Dub.,Streblus asper Lour,Syzygium cumini L.,Tamarindus indica L., andZizyphus oenoplia L., from the areas around the Gujarat State Fertilizer Company, near Baroda (22°−30′ N latitude, 73°−60′ E longitude), and nearly-normal area of Vallabh Vidyanagar (22°−50′ N latitude, 73°−10′ E longitude). The soil types, temperature, rainfall, humidity and agricultural patterns of the two localities are identical. Intensities of enzyme activity vary in different cell types of the epidermis and in various tissues of the stem. Intensities of succinate dehydrogenase and peroxidase in epidermal cells is low in majority of the pollution-affected plants. Except inAzadirechta indica andManilkara hexandra, stem of all the other pollution-affected species showed lower succinate dehydrogenase activity than in the normal. Vascular parenchyma in the stems of pollution-affected plants ofAzadirechta indica andManilkara hexandra showed high succinate dehydrogenase activity than that in the normal, whereas in extravascular regions it is almost identical with that of the normal stem. Peroxidase activity in the stem of pollution-affected plants ofManilkara hexandra andStreblus asper is higher than that in normal, and in rest of the species it is lower than or almost the same as in the normal.
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References
Bhatia D S and Malik C P 1977 Histochemical studies in stomatal apparatus ofPhaseolus mungo Linn.,Lathyrus sativus Linn. andQuantia elator Mill;Folia Histochem.Cytochem. 15 315–322
Curtis C R, Howell R K and Kremer D F 1976 Suy bean peroxidases from ozone injury:Environ. Pollut. 11 189–194
Galston A W, Bonner J and Baker R S 1953 Flavoprotein and peroxidase as components of the IAA-oxidase system of peas;Arch. Biochem. Biophys. 42 456–470
Hall J L and Sexton R 1972 Cytochemical localization of peroxidase activity in root cells;Planta 108 103–120
Heck W W, Taylor O C and Heggestad H E 1973 Air pollution research needs: Harbaceous and ornamental plants and agriculturally generated pollutants;APCA J. 23 257–266
Hepler P K, Rice R M and Terranova W A 1972 Cytochemical localization of peroxidase activity in wound vessel members ofColeus;Can. J. Bot. 50 977–983
Jensen W A 1955 The histochemical localization of peroxidase in roots and its induction by indole acetic acid;Plant Physiol. 30 426–432
Keller T 1974 The use of peroxidase activity for monitoring and mapping air pollution areas;Eur. J. For. Pathol. 4 11–19
Lee T T 1967 Inhibition of oxidative phosphorelation and respiration by ozone in tobacco mitochondria;Plant Physiol. 42 691–696
Mac Dowell F D H 1965 Stages of ozone damage to respiration of tobacco leaves,Can. J. Bot. 43 419–427
Maier R 1978 Activity and multiple forms of peroxidase inZea mays andMedicago sativa treated and non-treated with lead;Phyton 19 83–96
Mapson L W and Wardale D A 1971 Enzyme involved in the synthesis of ethylene from methionine or its derivatives in tomato;Phytochemistry 10 29–39
Mc Laughlin Jr S B and Barnes R 1975 Effects of fluoride on photosynthesis and respiration of some southeast American forest trees;Environ. Pollut. 8 91–96
Molner J M and La Croix L J 1972 Studies of the rooting of cuttings ofHydrangia macrophylla: enzyme changes;Can. J. Bot. 50 315–322
Nachlas M M, Tsow K C, De Souza E, Cheug C S and Seligman A M 1957 Cytochemical demonstration of succinic dehydrogenase by the use of a new-P-Nitrophenyl substituted ditetrazole;J. Histochem. Cytochem. 5 420–436
Pell E J and Brennan E 1973, Changes in respiration, photosynthesis, adenosine 5′-triphosphate and total adenylate content of ozonated pinto bean foilage as they relate to symptom expression;Plant Physiol. 51 378–381
Poovaiah B W and Rasmussen H P 1974 Localization of dehydrogenase and acid phosphatase in the abscission zone of bean leaves;Am. J. Bot. 61 68–73
Ridge I and Osborne D J 1970 Hydroxy proline and peroxidase in cell walls ofPisum sativum, regulation by ethylene.J. Expt. Bot. 21 853–856
Sakunthala Devi G 1981Phenological and ultrastructural studies in some angiosperm species growing around fertilizer complex, Ph.D. Thesis, Sardar Patel University, Gujarat
Sakunthala Devi G and Patel J D 1983 Variations in vegetation growing near a fertilizer complex. II. Chorophyll, sugar and protein contents;Biol. Bull. India 5 229–235
Seevers P M, Daly J M and Catedral F F 1971 The role of peroxidase enzymes in resistance to wheat stem rust desease;Plant Physiol. 43 353–360
Siegal S M 1962The plant cell wall (London: Pergamon)
Yung S F 1967 Biosynthesis of ethylene; ethylene formation from methionol by horse-radish peroxidase;Arch. Biochem. Biophys. 122 481–487
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Devi, G.S., Patel, J.D. Succinate dehydrogenase and peroxidase activities in leaf epidermis and stem of some angiosperm species growing near a fertilizer complex. Proc. Indian Acad. Sci. 95, 129–138 (1985). https://doi.org/10.1007/BF03053127
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DOI: https://doi.org/10.1007/BF03053127