Abstract
The effect of various factors uponin vitro germination ofAmaryllis vittata pollen was studied to find out optimum cultural requirements for obtaining high percentage of pollen germination and maximum tube growth. Temperature below −10°C was suitable for the storage ofAmaryllis pollen. Maximum germination was observed in sucrose medium. Pollen germination and the tube growth were maximal at the culture density of 5 mg/ml of the culture medium. The optimum temperature was 28±2°C. Studies on respiration suggested that optimum conditions for maximum O2 uptake were the same as for optimum pollen germination and pollen tube growth rate.
Similar content being viewed by others
References
Calzoni G L, Speranza A and Bagni N 1979In vitro germination of Apple pollens;Sci. Hort. 10 49–55
Dhaliwal A S and Malik C P 1980 Effect of Relative Humidity and CO2 on the shape, volume and fresh weight ofBrassica campestris L. pollenin vitro;Indian J. Exp. Biol. 18 1522–1523
Dickinson D B 1978 Influence of borate and pentaerythritol concentration on germination and tube growth ofLilium longiflorum pollen;J. Amer. Soc. Hort. Sci. 103 413–416
Nygaard P 1969 Studies on the germination of pine pollen(Pinus mugo) in vitro I. Growth conditions and effect of pH and temperature on germination, tube growth and respiration;Physiol. Plant 22 338–346
Nygaard P 1970 Studies on the germination of pine pollenin vitro II. Effect of different ions;Physiol. Plant 23 372–384
Sfakiotakis E M, Simon D H and Dilley D R 1972 Pollen germination and tube growth dependent on CO2 and independent of ethylene;Plant Physiol. 49 963–967
Sfakiotakis E M 1978 Germinationin vitro of Carob (Ceratonia siliqua L.) pollen;Z. Pflanze Physiol. 79 443–48.
Sharma Savita, Singh M B and Malik C P 1981 Dark CO2 fixation during germination ofAmaryllis vittata pollen in suspension cultures;Indian J. Exp. Biol. 19 710–714
Simons D H, Sfakiotakis E and Dilley D R 1972 Enhancement ofin vitro germination of lily with increased pre-inoculation humidity;Hort. Sci. 7 556–557
Stuart R and Street H E 1969 Studies on the gowth in culture of plant cells. IV. The initiation of division in suspension of stationary phase cells ofAcer. pseudoplatanus L.J. Exp. Bot. 20 556–71
Stuart R and Street H E 1971 Studies on the growth in culture of plant cells. X. Further studies on the conditioning of culture media by suspension ofAcer pseudoplatanus cells;J. Exp. Biol. 22 96–106
Tanaka K 1955 The pollen germination and pollen tube development inPinus densiflora Sieb; et. Zucc. III. The growth-inhibiting substances in ether, extracts fromPinus pollen grain;Sci. Res. Tohoku Univ. (Biol)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sharma, S., Malik, C.P. & Singh, M.B. Optimum cultural requirements forin vitro germination ofAmaryllis vittata Ait (Amaryllidaceae) pollen. Proc. Indian Acad. Sci. 90, 363–369 (1981). https://doi.org/10.1007/BF03052934
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF03052934