Summary
A pure culture of the fungusCorticium microsclerotia (Matz) Weber, obtained from the Centraalbureau voor Schimmelcultures, Baarn, Holland, was found to be thiamine heterotrophic, when cultured on a glucose nitrate medium.
Under the cultural conditions employed the fungus was observed to reach its maximum growth in about 15 days, in the presence of an optimum amount of 2·0 µg. of thiamine per flask, containing 20 ml. of medium. The fungus did not require the intact thiamine molecule as such and grew almost equally well when supplied with an equimolar mixture of thiazole and pyrimidine. The fungus also had the ability to slowly complete the synthesis of thiamine from pyrimidine alone, but not from thiazole.
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Communicated by Prof. T. S. Sadasivan,m.sc.,ph.d., f.a.sc.
An erratum to this article is available at http://dx.doi.org/10.1007/BF03050363.
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Mathew, K.T. Thiamine, its intermediates and growth ofCorticium microsclerotia (Matz) Weber. Proc. Indian Acad. Sci. 38, 1–9 (1953). https://doi.org/10.1007/BF03051161
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DOI: https://doi.org/10.1007/BF03051161