Summary
1. The common organic acids inhibited leaf phosphatase activity, This effect is mostly due to the hydroxyl groups in them.
2. The less common organic acids, which have only carboxyl groups, did not show any marked inhibitory action on phosphatase activity.
3. The less common organic acids eluted the leaf phosphatase after adsorption on aluminacγ gel to a greater extent than the more common organic acids.
4. The second elution of the purified enzyme from the aluminacγ gel was not possible with the organic acids as it was adsorbed on the gel.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bertho, A. and Grassmann, W.Biochemisches Praktikum, Walter de Gruyter and Co., Berlin, 1936.
Das, R. and Giri, K. V...Ann. Biochem. Exptl. Med., 1945,5, 63.
Fiske, C. H. and Subbarow, Y.J. biol. Chem., 1925,66, 375.
Franzen, H. and Helwert, F...Biochem. Z., 1923,135, 384.
King, E. J. and Abul-Fadl, M. A. M.Biochem. J., 1948,42, 28.
Meyer, A...Landw. Vers. Sta., 1887,34, 127.
Naganna, B. and Sripathi, C. E.Nature, Lond., 1954,174, 593.
Nelson, E. K. and Mottern, H. H.J. Amer. Chem. Soc., 1931,53, 3046.
Suzuki, Hoshimura, and TakaishiTokyo Imp. Univ. Coll. Agr. Bull., 1907,1, 503.
Author information
Authors and Affiliations
Additional information
Communicated by Prof. P. S. Sarma,f.a.sc.
Rights and permissions
About this article
Cite this article
Rao, J.J. Effect of some organic acids on leaf phosphatase from French beans. Proc. Indian Acad. Sci. 62, 149–154 (1965). https://doi.org/10.1007/BF03051088
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03051088