Summary
1. Fixation for sixty to ninety minutes in Gram’s iodine diluted with an equal quantity of water and incorporated with formaldehyde and glacial acetic acid gives a life-like preservation of the cellular structures except for the overall reduction in the size of the cells.
2. The necessity for the removal of RNA by acid hydrolysis as a preliminary but essential step to the succeeding staining procedures is emphasized. Judged from stained preparations there is little difference between extraction of RNA with normal HCl at 60° C. or with 10% perchloric acid at 28° C.
3. The structure of the nucleus in carefully stained hæmatoxylin preparations are superposable on those seen in living cells under phase contrast. While euco-basic fuchsin tints only a limited region of the nucleus, the details revealed by the Giemsa stain are intermediate between Feulgen and hæmatoxylin preparations.
4. The nucleus occupies an extra-vacuolar position. The vacuole is Feulgen negative and is therefore a cytoplasmic inclusion.
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Communicated by Dr. M. K. Subramaniam,f.a.sc.
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Royan, S. Evaluation of staining procedures following fixation of yeast in iodine formol acetic solution. Proc. Indian Acad. Sci. 47, 31–42 (1958). https://doi.org/10.1007/BF03051041
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DOI: https://doi.org/10.1007/BF03051041