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Studies on an enzyme reacting with isoniazid fromMycobacterium tuberculosis H37Rv

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Proceedings / Indian Academy of Sciences

Abstract

Mycobacterium tuberculosis H37Rv possesses an enzyme (referred to as ‘Y enzyme’) which catalyses in the presence of INH and NAD, the formation of a product, which turns yellow on acidification. The requirements for the reaction, such as enzyme concentration, INH concentration, etc., have been standardized. The substrate specificity of the enzyme with respect to INH and NAD has been determined. The reaction is specific for the INH-sensitive strain and is totally absent in INH-resistant strains. Furthermore, the ‘Y enzyme’ shows some characteristic features of a peroxidase in its requirement for oxygen and sensitivity to inhibition by various reagents. The requirements of this enzyme which is involved in the action of isoniazid inM. tuberculosis H37Rv is described for the first time.

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Abbreviations

INH:

isonicotinic acid hydrazide (isoniazid)

NAD:

nicotinamide-adenine dinucleotide

NADH:

reduced form of NAD

NADP:

nicotinamide-adenine dinucleotide phosphate

NADPH:

reduced form of NADP

NMN:

nicotinamide mononucleotide

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Authors and Affiliations

  1. Microbiology and Pharmacology Laboratory, Indian Institute of Science, 560012, Bangalore

    B. Gayathri Devi, T. Ramakrishnan F.A.Sc. & K. P. Gopinathan

Authors
  1. B. Gayathri Devi
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  2. T. Ramakrishnan F.A.Sc.
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  3. K. P. Gopinathan
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Gayathri Devi, B., Ramakrishnan, T. & Gopinathan, K.P. Studies on an enzyme reacting with isoniazid fromMycobacterium tuberculosis H37Rv. Proc. Indian Acad. Sci. 80, 240–252 (1974). https://doi.org/10.1007/BF03050671

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  • DOI: https://doi.org/10.1007/BF03050671

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