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Polygalacturonase activity ofAspergilli

  • Published:
Proceedings / Indian Academy of Sciences

Summary

  1. 1.

    Three strains ofAspergilli having good PG activity were isolated.

  2. 2.

    The conditions for maximum elaboration of PG were studied using synthetic liquid media as well as wheat bran.

  3. (i)

    The enzyme content was maximum after 4 days of growth; pH below 5 favoured the formation of enzyme.

  4. (ii)

    Wheat bran was a better substrate than synthetic liquid media; aqueous glycerine was very satisfactory for extraction of the enzyme from wheat bran medium.

  5. 3.

    Partially purified PG preparations were obtained by precipitating enzyme at 60 per cent. concentration of ammonium sulphate after discarding the precipitate at 20 per cent. salt concentration; alcohol inactivated the enzyme to a great extent.

  6. 4.

    The optimum pH for activity with citrus pectin as substrate was about 4–5; hydrolysis was rapid during first 12–24 hours and decreased thereafter during a further 8–10 days period.

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References

  1. Kertesz, Z. I., in Nord, F.F. and Weidenhagen, R.Ergebn. Enzymforsch, 1936,5, 233.

    CAS  Google Scholar 

  2. Rogers, H.J. Biochem. J., 1946,40,Proc. XVII.

  3. McColloch, R. J. and Kertesz, Z. I.J. Biol. Chem., 1945,160, 649.

    Google Scholar 

  4. Jansen, E. F. and MacDonnell, L. R.Arch. Biochem., 1945,8, 97.

    CAS  Google Scholar 

  5. Ehrlich, F.Enzymologia, 1937,3, 185.

    CAS  Google Scholar 

  6. Harter, L. and Weimer, J.J. Agri. Research, 1921–23,21, 609;22, 371;24, 861;25, 155.

    CAS  Google Scholar 

  7. Willaman, J. J. and Kertesz, Z. I.N. Y. State Agr. Expt. Sta. Bull., 1931, No. 178.

  8. Waksman, S. A. and Allen, M. C.J. Am. Chem. Soc., 1933,55, 3408.

    Article  CAS  Google Scholar 

  9. Phaff, H. J.Arch. Biochem., 1947,13, 67.

    CAS  Google Scholar 

  10. Gaumann, E. and Bohni, E.Helv. Chem. Acta., 1947,30, 24 and 1591.

    Article  CAS  Google Scholar 

  11. Beaven, G. H. and Brown, F.Biochem. J., 1949,45, 221.

    CAS  Google Scholar 

  12. Kertesz, Z. I.J. Am. Chem. Soc., 1939,61, 2544.

    Article  CAS  Google Scholar 

  13. Dickson, A. D., Otterson, H. and Link, K. P. —, 1930,52, 775.

    Article  CAS  Google Scholar 

  14. Clark, F. P.J.A.O.A.C., 1932,15, 136.

    CAS  Google Scholar 

  15. Fish, V. B. and Dustman, R. B.J. Am. Chem. Soc., 1945,67, 1155.

    Article  CAS  Google Scholar 

  16. Rietz, E. and Maclay, W. D. —, 1943,65, 1242.

    Article  CAS  Google Scholar 

  17. Willstätter, R. and Schudel, G.Ber. deut. Chim. Ges., 1918,51, 780.

    Article  Google Scholar 

  18. Kertesz, Z. I.J. Biol. Chem., 1935,108, 127.

    CAS  Google Scholar 

  19. Prescott, S. C. and Dunn, C.G.Industrial Microbiology, 1940, p. 344.

  20. Jones, D. R.Nature, 1946,158, 625.

    Article  Google Scholar 

  21. Takamine, J.Ind. Eng. Chem., 1914,6, 824; U.S. Patent 1, 460, 736 (1923).

    Article  CAS  Google Scholar 

  22. Underkofler, L. A., Severson, G. M. and Goering, K. J.Ind. Eng. Chem., 1946,38, 980.

    Article  CAS  Google Scholar 

  23. ——— and Christensen, L. M.Cereal Chem., 1947,24, 1.

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Chemical Technology, University of Bombay, India

    M. S. Pavgi, H. R. Pithawala, G. R. Savur & A. Sreenivasan

Authors
  1. M. S. Pavgi
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  2. H. R. Pithawala
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  3. G. R. Savur
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  4. A. Sreenivasan
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Pavgi, M.S., Pithawala, H.R., Savur, G.R. et al. Polygalacturonase activity ofAspergilli . Proc. Indian Acad. Sci. 34, 33–42 (1951). https://doi.org/10.1007/BF03050556

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  • DOI: https://doi.org/10.1007/BF03050556

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