Summary
The ability of strains of representative species of sporeforming bacilli to sporulate endotrophically in distilled water has been demonstrated. The degree of sporulation which a bacterium may undergo is a strain specific character but some species are more capable than others. The effect of various exogenous compounds on the phenomenon has been studied. It has been observed that various metallic salts, organic acids and autoclaved carbohydrates inhibit sporulation at concentrations that are growth inhibitory in nutrient media. It has been postulated that all or most growth inhibitory substances may at similar or lower concentrations inhibit endotrophic sporulation. A hitherto accepted procedure of sterilisation, the autoclaving of concentrated aqueous solutions of sugars, has been found to enhance the ability of the sugar to inhibit sporulation. None of the growth factors tested had any effect on sporulation.
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References
Amaha, M., Ordal, J. Z. and Touba, A. “Sporulation requirements ofB. coagulans var.thermoacidurans in complex media,”J. Bact., 1956,72, 34.
Bhat, J. V. and Iyer, V... “Aerobic mesophilic sporeforming bacteria in Indian environments,”Proc. Ind. Acad. Sci., 1955,42 B, 325.
Curran, H. R. and Evans, F. R. “The influence of iron and manganese upon the formation of spores by mesophilic aerobes in fluid organic media,”J. Bact., 1954,67, 489.
Foster, J. W. and Heiligman, F. “Mineral deficiencies in complex organic media as limiting factors in sporulation,” Ibid., 1949,57, 613.
Hachisuka, Y., Amaho, N., Kato, N. and Kuno, T. “About the nitrogen source required in development of germination,”Japanese Jour. Bact., 1954,9, 1129.
Hardwick, W. A. and Foster, J. W. “Enzymatic changes during sporogenesis in some aerobic bacteria,”J. Bact., 1953,65, 355.
Iyer, V. and Bhat, J. V... “Thermal resistance of certain dominant aerobic bacilli,”J. Sci. and Industr. Res., 1952,119, 427.
Kanga, D. H. .. “Studies on the growth and sporulation of sporeforming bacilli in defined media,”M.Sc. Thesis, Gujarat University, 1959.
Krishna Murthy, G. G. and Halvorson, H. O. “Effect of enzyme inhibitors on germination of and growth fromB. cereus,”J. Bact., 1957,73, 230.
Perry, J. J. and Foster, J. W... “Intracellular events occurring during endotrophic sporulation ofB. mycoides,” Ibid., 1954,67, 295.
Roberts, J. L. and Baldwin, I. L. “Spore formation byB. subtilis in peptone solution altered by activated charcoal,” Ibid., 1942,44, 653.
Weinberg, E. D... “Effect of manganese and antimicrobial drugs on sporulation ofB. subtilis in nutrient broth,” Ibid., 1955,70, 289.
Williams, O. H. and Lamanna, C. “Symposium on the biology of bacterial spores,”Bact. Revs., 1952,16, 89.
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Communicated by Dr. J. V. Bhat,f.a.sc.
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Iyer, V., Kanga, D.H. Factors influencing endotrophic sporulation in aerobic sporeforming bacilli. Proc. Indian Acad. Sci. 51, 133–142 (1960). https://doi.org/10.1007/BF03050018
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DOI: https://doi.org/10.1007/BF03050018