Abstract
Clearing can be successfully attained by soaking portions of leaves in a mixture of trichloroacetic acid and phenol (2:1) for 10–15 min at 60° C. These can be stained with writing ink (Chelpark make). The intensity of staining does not change even in permanent preparations. The preparations made as outlined above have been found specially beneficial for studying the morphology of fungi parasitic on leaves.
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Ram, H.Y.M., Nayyar, V.L. A leaf-clearing technique with a wide range of applications. Proc. Indian Acad. Sci. 87, 125–127 (1978). https://doi.org/10.1007/BF03046963
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DOI: https://doi.org/10.1007/BF03046963