Abstract
The carbon and nitrogen nutrition ofClaviceps microcephala, isolated from the ear heads ofPennisetum typhoides, was studied at 24°C for 20 days at pH 6·0. Out of forty-one carbon compounds tested, the pathogen showed excellent growth on dextrose, sucrose, pectin and methyl alcohol; good on mannose, fructose and maltose; fair on galactose, cocount oil and isopropyl alcohol; and poor or no growth on rest of the carbon compounds. Out of thirty-three nitrogenous compounds tested, the pathogen showed excellent growth on casein hydrolysate, yeast extract, asparagine, peptone, proline, glutamic acid and aspartic acid; good on ammonium nitrate and ammonium oxalate; fair on ammonium chloride, ammonium sulphate, arginine mono HCl and ammonium phosphate; and poor or no growth on rest of the nitrogen compounds. In general, compounds which supported best mycelial growth ofC. microcephala yielded its excellent sporulation andvice versa.
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References
Agarwal, G. P... “Nutritional studies onCurvularia penniseti. II. Carbon and nitrogen requirements,”Phyton, 1958,11, 143–51.
Alderman, D. J. and Jones, E. B. G. “Physiological requirements of two marine Phycomycetes,Althornia crouchii andOstracoblabe implexa,”Trans. Br. mycol. Soc., 1971,57, 213–25.
Bais, B. S., Singh, S. B. and Singh, D. V. “Effect of different carbon and nitrogen compounds on the growth and sporulation ofCurvularia pallescens,”Indian Phytopath., 1970,23, 511–17.
Bhargava, S. N... “Nitrogen requirements of three storage rot fungi,”Sydowia, Annales Mycologici Ser. II, 1970,24, 308–16.
—————.. “Carbon requirements of three storage rot fungi,”Proc. Nat. Acad. Sci., India, 1971,41 B, 367–72.
Chan, C. and Stephen, R. C... “Utilisation of different carbon sources by genusLinderina,”Canad. Jour. Bot., 1968,46, 751–56.
Cochrane, V. W... “The metabolism of species ofStreptomyces. III. The nitrate metabolism ofS. griseus,”Bull. Torrey Bot. Club, 1950,77, 176–80.
Cochrane, V. W...Physiology of fungi, John Wiley and Sons, Inc., New York, 1958.
—— and Conn, J. E... “The metabolism of species ofStreptomyces. II. The nitrate metabolism ofS. coelicolor,”Bull. Torrey Bot. Club, 1950,77, 10–18.
Fergus, C. L... “The nutrition ofPenicillium digitatum Sacc.,”Mycologia 1952,44, 183–99.
Goldstein, S... “Development and nutrition of new species ofThraustochytrium,”Amer. Jour. Bot., 1963,50, 271–79.
Hasija, S. K... “Physiological studies ofAlternaria citri andA. tenuis,”Mycologia, 1970,62, 289–95.
Howard, K. L. and Howard, E. B. “Nutritional studies on two Gasteromycetes:Phallus ravenelii andCrucibulum levis,” Ibid., 1969,61, 606–13.
Kurtz, E. B. and Fergus, C. L. “Nitrogen nutrition ofColletotrichum coccodes,”Phytopathology, 1964,54, 691–92.
Landers, K. E. “Growth ofCercospora arachidicola in glucose—phosphate—asparagine—thiamine agar medium,” Ibid., 1964,54, 1236–39.
Lewis, R. W... “Amino acid nutrition ofAlternaria solani,” Ibid., 1957,47, 121–25.
Lilly, V. G. and Barnett, H. L.The Physiology of Fungi, McGraw-Hill Company, Inc., New York, 1951.
—————.. “The utilisation of sugars by fungi,”West Va. Univ. Agric. Expt. Sta. Bull., 1953,362 T, 5–58.
Mandahar, C. L... “Nutritional studies ofPleospora indica,”Res. Bull. Panjab Univ., 1965,16, 305–12.
Matsushima, T. and Klug, R. J. “Utilisation of L-sorbose by monosporidial lines and mutants ofUstilago maydis,”Amer. Jour. Bot., 1958,45, 165–68.
Murray, S. C. and Andrian, M. “Physiological and morphological studies of a morphological mutant ofNeurospora crassa stimulated by sorbose,”Bot. Gaz., 1960,122, 72–76.
Sarbhoy, A. K... “Nutritional studies on some members of the Mucorales V. I. Utilisation of different nitrogen compounds,”Path. Microbiol., 1965,28, 816–29.
Schade, A. L... “The nutrition ofLeptomitus,”Amer. Jour. Bot., 1940,27, 376–84.
Sen, B. and Munjal, R. L... “Studies on the physiology ofUstilago nuda tritici Schaf.,”Indian Phytopath., 1968,21, 416–22.
Singh, R. S. and Khanna, R. N. “Physiological studies onAlternaria tenuis Auct., the fungu causing a black core rot of Mandarin oranges,”Jour. Indian Bot. Soc., 1966,45, 277–86.
Swack, N. S. and Miles, P. G. “Conditions affecting growth and indigotin production by strain 130 ofSchizophyllum commune,”Mycologia, 1960,52, 574–83.
Tandon, R. N. and Chandra, S. “The nutrition ofColletotrichum gleosporioides Penz.,”Mycopath. et Myco. App., 1962,18, 213–24.
Thind, K. S. and Madan, M. “The effect of various carbon sources on the growth and sporulation ofCephalothecium roseum Corda causing pink rot of apple (Malus sylvestris Mill.),”Proc. Nat. Acad. Sci. India, 1967,37 B, 373–80.
————— “The effect of various nitrogen sources on the growth and sporulation ofCephalothecium roseum Corda causing pink rot of apple (Malus sylvestris Mill.),” Ibid., 196939 B, 233–40.
————— “The influence of different sources of carbon on the growth ofCercospora spp.,” Ibid., 1964,34 B, 387–93.
Yusef, H... “The requirements of some Hymenomycetes for essential metabolites,”Bull. Torrey Bot. Club, 1953,80, 43–64.
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Thind, K.S., Madan, M. Effect of various carbon and nitrogen sources on the growth and sporulation ofClaviceps microcephala . Proc. Indian Acad. Sci. 78, 241–256 (1973). https://doi.org/10.1007/BF03045605
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DOI: https://doi.org/10.1007/BF03045605