Zusammenfassung
□ Bei Untersuchungen zur wachstumsabhängigen Genregulation konnten wir aus einer Pankreaskarzinomzellinie eine Gruppe Zinkfingermotive, Ankyrindomänen und Pleckstrinhomologien enthaltender Proteine (ZAP) klonieren. Beispiele der funktionellen Bedeutung dieser Domänen finden sich in Proteinen, die an verschiedenen Signaltransduktionskaskaden und der Regulation des Zellzyklus beteiligt sind. Weiterhin konnten für die einzelnen ZAP-Proteine spezifische Funktionsmotive, ihre chromosomale Lokalisation und gewebespezifische Transkription charakterisiert werden. Die bisher ermittelten Daten deuten auf ein breites Spektrum potentieller Protein-Protein-Interaktionen hin. Spezifische Interaktionspartner sind zum jetzigen Zeitpunkt noch nicht identifiziert.
□ Zusammenfassend könnte es sich aufgrund der Vielzahl von sehr gut definierten Proteindomänen und der transkriptionellen Daten bei den Mitgliedem der ZAP-Familie um Scaffold-Proteine im komplexen Netzwerk wachstums-assoziierter intrazellulärer Signaltransduktionskaskaden handeln.
Abstract
□ While interested in proliferation-dependent gene regulation in a pancreatic carcinoma cell line, we cloned a set of proteins (ZAP) characterized by a conserved region consisting of consecutive zinc finger, ankyrin repeat and PH domains. Functional aspects of these domains were obtained by comparison with proteins involved in several signal transduction pathways and cell cycle regulation. The members of the ZAP protein family are individually characterized by different types of supplementary protein family are individually characterized by different types of supplementary protein domains, their chromosomal localization and their tissue specific gene transcription. All results indicate a wide spectrum of protein-protein interactions. Up to now specific binding partners have not been identified.
□ In summary, the multiplicity of conserved regions and transcriptional data indicate a scaffold function for ZAP proteins in the complex network of proliferation associated intracellular signal transduction pathways.
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Günther, R., Zill, H., Schmidt, W.E. et al. ZAP-Gene: Charakterisierung der Proteinstruktur einer neuen Familie proliferationsassoziierter Gene im exokrinen Pankreas. Med Klin 94, 233–238 (1999). https://doi.org/10.1007/BF03044861
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DOI: https://doi.org/10.1007/BF03044861