Zusammenfassung
Die Möglichkeiten der Ausbreitung von Transgenen durch sexuellen oder horizontalen Gentransfer wurden am Beispiel von Freilandversuchen mit Herbizid-resistentem Raps in Rheinland-Pfalz untersucht. In Abhängigkeit von Klimaund Wetterbedingungen sowie der Größe der Anbaufläche kann beim Raps eine Auskreuzung transgener DNA auf nicht-transgenem Raps oder verwandte Wildformen stattfinden. Die Raps-DNA (und damit auch daspat-Gen) persistiert über Monate hinweg im Boden und steht damit prinzipiell für eine Aufnahme durch Mikroorganismen zur Verfügung. Eine häufige Integration in das Genom von Bakterien oder gar die Realisierung der aufgenommenen genetischen Information ist jedoch wenig wahrscheinlich.
Summary
The possibilities of the extension of transgenic DNA by sexual or horizontal gene transfer were investigated during experiments with herbicide-resistant rape on a field in Rheinland-Pfalz. Depending on climate and weather conditions as well as on the size of the area for the release the pollination of non-trangenic rape or wild relatives can occur by outcrossing of the transgenic rape-DNA. The rape-DNA (as well as thepat-gene) persists in the soil during months and can be taken up on principle by microorganisms. An abundant integration, however, into the genome of bacteria or the expression of the integrated genetic information is not very like. *** DIRECT SUPPORT *** A06PF023 00004
Literatur
Rissler, I., and Mellon, M.: Perils amidst the promise. Ecological risks of transgenic crops in a global market. Union of Concerned Scientists. Cambridge, MA (1993).
Klehr-Wirth, D., Wirth, M., Grannemann, R., Münk, C., and Hauser, H.: Accidental formation of replication-competent viruses from gene transfer by retroviral vectors. In: Schmidt, E. R., and Hankeln, T. (Hrsg.): Transgenic Organisms and Biosafety. Horizontal Gene Transfer, Stability of DNA and Expression of Transgenes. Heidelberg: Springer-Verlag 1996, 105.
Gould, F., Anderson, A., Jones, A., Sumerford, D., Heckel, D., Lopez, J., Micinski, S., Leonard, R., and Laster, M.: Initial frequency of alleles for resistance toBacillus thuringiensis toxins in field populations ofHeliothis virescens. Proc. Natl. Acad. Sci. USA94 (1997) 3519.
Kidwell, M. G.: Lateral transfer in natural populations of eukaryotes. Ann. Rev. Genet.27 (1993) 235.
Syvanen, M.: Horizontal gene transfer: evidence and possible consequences. Ann. Rev. Genet.28 (1994) 237.
Rogers, H. J., and Parkes, H. C.: Transgenic plants and the environment. J. Exp. Bot.46 (1995) 467.
Eckelkamp, C., Jäger, M., und Tappeser, B. Verbreitung und Etablierung rekombinanter DNA in der Umwelt. Studie des Öko-Institutes Freiburg, im Auftrag des Umwelbundesamtes Berlin (1998) (im Druck).
Danneberg, G., und Driesel, A. J.: Verbreitung und Etablierung rekombinanter DNA in der Umwelt. Studie des TÜV Energie und Systemtechnik, Eschborn, im Auftrag des Umweltbundesamtes Berlin (1998) (im Druck).
Gebhard, F., and Smalla, C.: Transformation ofAcinetobacter sp. strain BD413 by transgenic sugar beet DNA. Appl. Env. Microbiol.64 (1998) 1550.
Schlüter, K., Fütterer, J., and Potrykus, I.: «Horizontal» gene transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysantemum) occurs — if at all — at an extremely low frequency. Bio/Technology13 (1995) 1094.
Tiedje, J. M., Colwell, R. K., Grossman, Y. L., Hodson, R. E., Lenski, R. E., Mack, R. N., and Regal, P. J.: The planned introduction of genetically engineered organisms: ecological considerations and recommendations. Ecol.70 (1989) 298.
Manasse, R., and Kareiva, P.: Quantifying the spread of recombinant genes and organisms. In: Ginzburgh, L. (ed.): Assessing ecological risks of biotechnology. Boston: Butterworth, Heinemann, 1991, 215.
Kerlan, M. C., Chevre, A. M., Eber, F., Baranger, A., and Renard, M.: Risk assessment of outcrossing of transgenic rapeseed to related species: 1. Interspecific hybrid production under opitmal conditions with emphasis on pollination and fertilization. Euphytica62 (1992) 145.
Scheffler, J. A., Parkinson, R., and Dale, P. J.: Frequency and distance of pollen dispersal from transgenic oilseed rape (Brassica napus). Transgenic Res.2 (1993) 356.
Kareiva, P., Morris, W., and Jacobi, C. M.: Studying and managing the risk of cross-fertilization between transgenic crops and wild relatives. Mol. Ecol.3 (1994) 15.
Linder, C. R., and Schmitt, J.: Assessing the risk of transgene escape through time and crop-wild hybrid persistence. Mol. Ecol.3 (1994) 23.
Wilkinson, M. J., Timmons, A. M., Charters, V., Dubbels, S., Robertson, A., Wilson, N., Scott, S., O’Brien, E., and Lawson, H. M.: Problems of risk assessment with genetically modified oil seed range. Brighton Crop Protection Conference Weeds (1995) 1035.
Timmons, A. M., O’Brien, E. T., Charters, Y. M., Dubbels, S. J., and Wilkinson, M. J.: Assessing the risks of wind polination from fields of genetically modifiedBrassica napus ssp.oleifera. Euphytica85 (1995) 417.
Smalla, K., Gehard, F., van Elsas, J. D., Hatzk, J. D., and Schiemann, J.: Bacterial communities influenced by transgenic plants. In: Proceedings of the 3rd International Symposium on the Biosafety Results of Field Tests of Genetically Modified Plants and Microorganisms, Monterey, CA (1994) 157.
Broer, I., Dröge-Laser, W., and Gerke, M.: Examination of the putative horizontal gene transfer from transgenic plants to Abgobacteria. In: Schmidt, E. R., und Hankeln, T. (eds.): Transgenic Organisms and Biosafety. Horizontal Gene transfer, Stability of DNA and Expression of Transgenes. Heidelberg: Springer-Verlag 1996, 67.
Mitten, D., Redenbaugh, K., and Lindemann, J.: Evaluation of potential gene transfer from transgenic plants. In: Schmidt, E. R., and Hankeln, T. (eds.): Transgenic Organisms and Biosafety. Horizontal Gene transfer, Stability of DNA and Expression of Transgenes. Heidelberg: Springer-Verlag 1996, 95.
Schiemann, J., Backhaus, H., Commandeur, U., Dietz-Pfeilstetter, A., Engelen, B., Gehard, F., Heuer, H., Koenig, R., Lesemann, D. E., Maiß, E., Matzk, A., Niepold, F., Smalla, K., K., and Casper, R.: New methods and results in monitoring field release of genetically modified organisms. In: The 4th International Symposium on the biosafety results of field tests of genetically modified plants and microorganisms, Japan (1977) 705.
Scheffler, J. A., and Dale, P. J.: Opportunities for gene transfer from transgenic oilseed rape (Brassica napus). Transgenic Res.3 (1994) 263.
Jørgensen, R. B., and Andersen, B.: Spontaneous hybridization between oilseed rape (Brassica napus) and weedyB. campestris (Brassicaceae): a risk of growing genetically modified oilseed rape. Am. J. Bot.81 (1994) 1620.
Rakow, G., and Woods, D. L.: Outcrossing in rape and mustard under Sasketchewan prairic conditions. Canad. J. Plant Sci.67 (1987) 147.
Stringham, G. R., and Downey, R. K.: Effectiveness of isolation distances in seed production of rape seed (Brassica napus). Agronomical Abstracts (1982) 136.
Crawley, M. J., Hails, R. S., Rees, M., Kohn, D., and Buxton, J.: Ecology of transgenic oilseed rape in natural habitats. Nature363 (1993) 620.
Fredshavn, J. R., Poulsen, G. S., Huybrechts, I., and Rüdelsheim, P.: Competitiveness of transgenic oilseed rape. Transgenic Res.4 (1995) 142.
Ahl-Goy, P., and Duesing, J. H.: Assessing the environmental impact of gene transfer to wild relatives. Bio/Technology14 (1996) 39.
Smalla, K., Cresswell, N., Mendonca-Hagler, L., Wolters, A., und van Elsas, I. D.: Rapid DNA extraction protocoll from soil for polymerase chain reaction mediated amplification. J. Appl. Bacteriol.74 (1993) 78.
Becker, J., Siegert, H., Logemann, J., und Schell, J.: Begleitende Sicherheitsforschung zur Freisetzung gentechnisch veränderter Petunien. In: Bundesministerium für Forschung und Technik (Hrsg.): Biologische Sicherheit. Forschung Biotechnologie 3 (1994) 563.
Paget, E., and Simonet, P.: On the track of natural transformation in soil. FEMS Microb. Ecol.15 (1994) 109.
Wackernagel, W.: The persistence of DNA in the environment and its potential for bacterial genetic transformation. In: Schmidt, E. R., and Hankeln, T. (eds.): Transgenic Organisms and Biosafety. Horizonal Gene transfer, Stability of DNA and Expression of Transgenes. Heidelberg: Springer-Verlag 1996, 137.
Blum, S. A. E., Lorenz, M. G., and Wackernagel, W.: Persistence of DNA in natural soils: Adsorption to particulate material provides protection against nucleolytic degradation. In: Schmidt, E. R., and Hankeln, T. (eds.): Transgenic Organism and Biosafety. Horizontal Gene transfer, Stability of DNA and Expression of Transgenes. Heidelberg: Springer-Verlag 1996, 147.
FDA-Bericht der Firma CALGENE: KanR-gene: safety and use in the production of genetically engineered plants. Request for advisory opinion. Calgene Inc., CA, USA (1990).
Koonin, E. V., and Mushegian, A. R.: Complete genome sequences of cellular life forms: glimpses of theoretical evolutionary genomics. Curr. Opin Genet. Dev.6 (1996) 757.
Smalla, K., van Overbeck, L.S., Pakall, R., and van Elsas, J. D.: Prevalence of nptII and Tn5 in kanamycin-resistant bacteria from different environments. FEMS Microbiol. Evol.13 (1993) 47.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hankeln, T., Feldmann, R.C. & Schmidt, E.R. Untersuchungen zum Gentransfer und zur Freisetzung von DNA aus trangenen Pflanzen. Bundesgesundheitsbl. 41, 542–547 (1998). https://doi.org/10.1007/BF03044274
Issue Date:
DOI: https://doi.org/10.1007/BF03044274