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Untersuchungen zum Gentransfer und zur Freisetzung von DNA aus trangenen Pflanzen

Investigations of the gene transfer and the release of DNA from transgenic plants

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Bundesgesundheitsblatt Aims and scope

Zusammenfassung

Die Möglichkeiten der Ausbreitung von Transgenen durch sexuellen oder horizontalen Gentransfer wurden am Beispiel von Freilandversuchen mit Herbizid-resistentem Raps in Rheinland-Pfalz untersucht. In Abhängigkeit von Klimaund Wetterbedingungen sowie der Größe der Anbaufläche kann beim Raps eine Auskreuzung transgener DNA auf nicht-transgenem Raps oder verwandte Wildformen stattfinden. Die Raps-DNA (und damit auch daspat-Gen) persistiert über Monate hinweg im Boden und steht damit prinzipiell für eine Aufnahme durch Mikroorganismen zur Verfügung. Eine häufige Integration in das Genom von Bakterien oder gar die Realisierung der aufgenommenen genetischen Information ist jedoch wenig wahrscheinlich.

Summary

The possibilities of the extension of transgenic DNA by sexual or horizontal gene transfer were investigated during experiments with herbicide-resistant rape on a field in Rheinland-Pfalz. Depending on climate and weather conditions as well as on the size of the area for the release the pollination of non-trangenic rape or wild relatives can occur by outcrossing of the transgenic rape-DNA. The rape-DNA (as well as thepat-gene) persists in the soil during months and can be taken up on principle by microorganisms. An abundant integration, however, into the genome of bacteria or the expression of the integrated genetic information is not very like. *** DIRECT SUPPORT *** A06PF023 00004

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Hankeln, T., Feldmann, R.C. & Schmidt, E.R. Untersuchungen zum Gentransfer und zur Freisetzung von DNA aus trangenen Pflanzen. Bundesgesundheitsbl. 41, 542–547 (1998). https://doi.org/10.1007/BF03044274

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