Abstract
This study was carried out at the histochemical, immunohistochemical and scanning electron microscopic levels to elucidate morphology, distribution of elastic fibers and extracellular matrices (type I and III collagens). Specimens were collected from 26 cadavers (18 males and 8 females; 50–90 years of age) which had no muscular disease in the human face.
-
1.
Observation by macroscopy: A tight attachment between skin and connective tissue was found in the frontal and inferior labial regions, but at the superior labial, orbital and modiolus regions showed loose attachment in the face skin.
-
2.
Observation by microscopy: The elastic fibers (EF) were located in the epithelium, intercellular substance, connective tissue near muscle fibers, and the perimysium and endomysium of the muscle fibers. In the thick intercellular substance the concentrated EF are found, and they varied in arrangement in each of five observed locations. In measurement locations, the cross-sectional area of muscle fiber was sharply decreased, and was especially remarkable in the modiolous portion.
-
3.
Observation by immunohistochemistry: Type I and III collegens were distributed throughout connective tissue. Particularly, type I collagen was localized in the connective tissue of the superior labial, modiolus and orbital regions, and type III was distributed on the connective tissue of all examined regions except for the frontal region.
-
4.
Observation by scanning electron microscopy: Numerous fibers were composed of irregularly arranged small and large fibrous bundles from the papillary dermis to intercellular substance. A few very fine irregularly arranged fibers were forming a network, and were found around large EF bundles. These large bundles were irregularly arranged to other collagenous complicated fibers in almost all examined regions.
Similar content being viewed by others
References
Ker RF, Bennett MB, Bibby SR, Kester RC and Alexander RMCN: The spring in the arch of human foot, Nature,325: 147–149, 1987.
Parker F: An electron microscope study of coronary arteries, Am J Anat,103: 247–259, 1958.
Karrer HE: Electron microscope study of developing chick embryo aorta, J Ultrastruct Res,4: 508–524, 1960.
Karrer HE: An electron microscope study of the aorta in young and in aging mice, J Ultrastruct Res,5: 1–27, 1961.
Haust MD, More RH, Bencosme SA and Balis JU: Elastogenesis in human aorta. An electronmicroscopic study, Exp Mol Pathol,4: 508–524, 1965.
Greenlee TK Jr and Ross R: The development of the rat flexor digital tendon, a fine structure study, J Ultrastruct Res,18: 354–376, 1967.
Dyer RF and Enna CD: Ultrastructural features of adult human tendon, Cell Tissue Res,168: 247–259, 1976.
Rizk NN: Stereoscopic visualization of various morphological types of collagenous fibres, Acta Anat,107: 424–429, 1980.
Banfield WG and Brindley DC: Preliminary observations on senile elastosis using the electron microscope, J Invest Dermatol,41: 9–17, 1963.
Low FN: Microfibrils; Fine filamentous components of the tissue space, Anat Rec,142: 131–137, 1962.
Greenlee TK Jr, Ross R and Hartman JL: The fine structure of elastic fibers, J Cell Biol,30: 59–71, 1966.
Ali AM, Sharawy M, O’Dell NL and Al-Behery G: Morphological alterations in the elastic fibers of the rabbit craniomandibular joint following experimentally induced anterior disk displacement, Acta Anat,147: 159–167, 1993.
Burgeson RE: New collagen, new concepts, Ann Rev Cell Biol,4: 551–577, 1988.
Vuorio E and Crombrugghe BD: The family of collagen genes, Ann Rev Biochem,59: 837–872, 1990.
Desaki J and Uehara Y: The overall morphology of neuromuscular junctions as revealed by scanning electron microscopy, J Neurocytol,10: 101–110.
Inoue T and Osatake H: A new drying method of biological specimens for scanning electron microscopy, The t-butyl alcohol freeze-drying method, Arch Histol Cytol,51: 53–59, 1988.
Ross R and Bornstein P: The elastic fiber I, The separation and partial characterization of its macromolecular components, J Cell Biol,40: 366–381, 1969.
Fullmer HM: A comparative histochemical study of elastic, pre-elastic and oxytalan connective tissue fibers, J Histochem Cytochem,8: 290–295, 1960.
Padykula HA and Herman E: The specificity of the histochemical method for adenosine triphosphatase, J Histochem Cytochem,3: 170–185, 1955.
Wattenberg LW and Leong JL: Effect of coenzyme Q and menadione on succinate dehydrogenase activity as measured by tetrazolium salt reaction, J Histochem Cytochem,8: 296–303, 1960.
Novikoff AB, Shin W and Drucker J: Mitochondrial localization of oxidative enzymes; Staining results with two tetrazolium salts, J Biophys Biochem Cytol,9: 47–61, 1961.
Berry DC and Wilkie JK: Lip and tongue behavior in relation to prosthetics, The Dental Practitioner,11: 334–340, 1961.
Yamaguchi H, Tanaka Y, Sueishi K, Sebata M, Uchiyama T, Saito C and Shigematsu T: Changes in oral functions and muscular behavior due to surgical orthodontic treatment, Bull. Tokyo Dent. Coll,35: 41–49, 1994.
Lott F and Levin B: Flange Technique; Anatomic and physiologic approach to increased retention, function, comfort, and appearance of dentures, J Pros Den,16: 394–413, 1966.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Omata, Y., Sato, I. & Sato, T. An anatomical study of the adult human face: Distribution of elastic fibers and collagen fibers in the skin and subcutaneus tissue. Shigaku = Odontology 85, 356–375 (1997). https://doi.org/10.1007/BF03039033
Issue Date:
DOI: https://doi.org/10.1007/BF03039033