Abstract
The purpose of this study was to determine the effect of disorders of parasympathetic innervation or trophic vessels on the parenchymal tissue of the submandibular gland. ICR mice underwent resection of the chorda tympani nerve or transection of the trophic vessels. After treatment, the submandibular glands were removed at intervals and processed for examination using light and electron microscopy.
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1.
From 1 week after nerve resection, the submandibular gland parenchymal tissue showed expanded blood vessels, infiltration of wandering cells and growth of connective tissue fibers, etc. Degeneration of the acinar cells extended from the periphery of the gland proper toward the inside, and by 3–4 weeks degeneration was evident over a wide area of the gland. Striated duct and granular convoluted tubule (GCT) cells showed slight damage. At 8 weeks, GCT cells and at 10 weeks acinar and intercalated duct-like cells had begun to increase in number. At 20 weeks, the glandular tissue showed almost normal parenchyma.
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2.
From 3 weeks after nerve resection, the submandibular gland proper showed a marked decrease in weight, but returned to approximately 70% of the original weight by 20 weeks.
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3.
At 1 hour after trophic vessel transection, acinar, striated duct and GCT cells showed vacuolation of the cytoplasm in addition to nuclear atrophy. At 9 hours, growth of connective tissue in the capsule and phagocytosis of collapsed cells by wandering cells were observed. At 18 hours, almost all parenchymal cells inside the gland proper had degenerated, and at 72 hours the gland proper finally disappeared.
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4.
Electron microscopy and TUNEL staining showed that critical injury of the parasympathetic nerve induced necrocytosis in the acinar and GCT cells, whereas serious damage to the trophic vessels caused an apoptosis-like cell death in these cells.
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References
Bernard C: Du rôle des actions reflexes paralysantes dans le phénoméne des sécretion. J Anat Paris,1: 137–146, 1864.
Langley JN: On the physiology of the salivary secretion, Part III The ‘paralytic’ secretion of saliva. J Physiol,6: 7192, 1885.
Langley JN: On the physiology of the salivary secretion, VI. Chiefly upon the connections of peripheral nerve cells with the nerve fibers which run to the sublingual and submaxillary glands, J Physiol Lond,11: 123–158, 1890
Maximou A: Beiträge zur Histologie und Physiologie der Speicheldrüsen, Arch mirk Anat,58: 1–134, 1901.
Maximou A: Die Veränderung der Speicheldrüsen nach Durchtrennung der Chorda tympani, Zbl Physiol,16: 249–254, 1900.
Emmelin N: Paralytic secretion of saliva, An example of supersensitivity after denervasion, Physiol Rev,32: 21–45, 1952.
Emmelin N and Stromblad R: Salivary secretion after section of the chorda tympani in nonanaesthetized dogs. Acta Physiol, Scand,30: 65–74, 1953.
Rawlinson HE: The changes in the alveolar and demilune cells of the simple and the stimulated paralytic submaxillary gland of the cat, J Anat,93: 201–216, 1959.
Snell RS: The histochemical appearances of cholinesterase in the parasympathetic nerves supplying the submandibular and sublingual salivary glands of the rat, J Anat Lond,92: 534–543, 1958.
Snell RS: The effect of preganglionic parasympathectomy on the structure of the submandibular and major sublingual salivary glands of the rat. Z Zellforsch,35: 686–696, 1960.
Tamalin A: Submaxillary gland recovery from obstruction, I. Overall changes and electron microscopic alteration of granular duct cell. J Ultrastruct Res,34: 276–287, 1971.
Tamalin A: Submaxillary gland recovery from obstruction, II. Electron microscopic alterations of acinar cells, J Ultrastruct Res,34: 288–302, 1971.
Hanks CT and Chaudhry AP: Regeneration of rat submandibular gland of partial extirpation following a light and electron microscopic study. Am J Anat,130: 195–208, 1971.
Nagato T and Nagaki M: Occurence of a third type of secretory cell in the acinus of the rat submandibular gland. Anat Rec,236: 427–432, 1993.
Jacoby F and Lesson CR: The postnatal development of the submaxillary gland, J Anat,93: 201–216, 1959.
Strum JM: Unusual peroxidase-positive granules in the developing rat submaxillary gland, J Cell Biol,51: 575–579, 1971.
Gresik EW: Postnatal developmental changes in submandibular glands of rats and mice. J Histochem Cytochem,28: 860–870, 1980
Yohro T: Development of secretory units of mouse submandibular gland, Z Zellforsch,110: 173–184, 1970.
Suzuki Y and Takeda M: Basal cells in the mouse olfactory epithelium after axotomy: immunohistochemical and electron-microscopic studies, Cell Tissue Res,266: 239–245, 1991.
Gratzner HG: Monoclonal antibody to 5-bromo-and 5-iodo deoxyuridine: A new reagent for detection of DNA replication, Science,218: 474–475, 1982
Hatakeyama S, Sashima M and Suzuki A: A sexual dimorphism of mucous cells in the submandibular salivary gland of rat, Arch Oral Biol,32: 689–693, 1987.
Qwarnstrom EE and Hand AR: A granular cell at the acinar-intercalated duct junction of the rat submandibular gland, Anat Rec,206: 181–187, 1983.
DM Chisholm and MM ADI: Cell proliferation and apoptosis in isoprenaline-induced sialosis in the rat submandibular glands. Int J Exp Path,76: 263–269. 1995.
Koseki C, Herzlinger D and Al-Awqati Q: Apoptosis in metanephric development. J Cell Biol,119: 1327–1333, 1992.
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Takahashi, Y., Kurabuchi, S. & Aiyama, S. Histological changes in the mouse submandibular gland subjected to parasympathetic nerve block or ischemia: Comparison between chorda tympani resection and trophic vessel transection. Shigaku = Odontology 86, 826–841 (1999). https://doi.org/10.1007/BF03039018
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DOI: https://doi.org/10.1007/BF03039018