Abstract
Aim
Further experiments were performed to explain a difference in chromosomal aberration yield found between samples cultivated immediately after fission neutron irradiation and samples which were cultivated with 96 h delay after irradiation.
Material and Method
Human peripheral blood samples were irradiated in mixed fission neutron/gamma field (1800 s) and biological effect assessed in the mean of analysis of unstable chromosome aberrations with a time delay in culturing cells of 12, 24, 48, and 96 h. Additional measurements were performed on irradiated and blank blood samples with the aim to detect any increase in α and β activity after fission neutron irradiation. No difference was found. Results were compared to theoretically calculated values of the α and β activity released from natural radioactive isotopes.
Result and Conclusion
As a conclusion it is shown that in our experimental conditions the secondary effects resulting from nuclear transformations of natural or induced radioactive isotopes, recoil reactions and accompanying α, β, and γ radiation are not the reason for the increase observed in chromosomal aberration yield in blood samples cultured with a time delay of at least 24 hours.
Zusammenfassung
Ziel
Um Differenzen in der Chromosomaberration zu erkennen, wurden weitere Experimente durchgeführt.
Material und Methode
Es wurden zwei Kulturen angelegt: unmittelbar nach der Bestrahlung mit Spaltneutronen und 96 Stunden nach der Bestrahlung. Die Proben von menschlichem peripherem Blut wurden in einem Mischfeld aus Neutronen-/Gammastrahlen für 1800 s bestrahlt und die biologischen Strahleneffekte mit Hilfe der instabilen Chromosomaberration nach einer Lagerung von 12, 24, 48 und 96 Stunden untersucht. Messungen an bestrahltem und nichtbestrahltem Blut zeigten keinen Anstieg der α- und β-Aktivität. Die Resultate wurden mit den theoretisch berechneten α- und β-Aktivitäten der natürlichen Isotopen verglichen.
Ergebnis und Schlußfolgerung
Das Ergebnis der Experimente zeigte, daß Sekundäreffekte, die aus der Kernumwandlung von natürlichen oder induzierten radioaktiven Stoffen resultieren, und die begleitende α- β- und γ-Strahlung nicht die Ursache für das beobachtete Ansteigen der Chromosomaberration in Blutproben, die nach mindestens 24 Stunden kultiviert wurden, sind.
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Fajgelj, A., Horvat, D. & Škrk, J. Chromosome aberrations induced in human lymphocytes by U-235 fission neutrons. Strahlenther. Onkol. 173, 91–97 (1997). https://doi.org/10.1007/BF03038928
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DOI: https://doi.org/10.1007/BF03038928