Abstract
Two toxicity tests (pollen tube growth test and methylen blue-assay) were examined regarding their sensitivity towards certain chosen mycotoxins. The IC50 values of the PTGT were in a concentration range between 0,01 μg/ml and 11,18 μg/ml, of the MB-assay in a concentration range between 0,003 μg/ml and 17,91 μg/ml. Both tests can be used to record toxigenic fungal wild strains in mould-infested apartments. Independently from the well-known immunochemical methods or HPLC/GC-MS analysis must be used for an exact determination of the kind and the content of the mycotoxins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Gareis M, Johannig E, Dietrich R (1999) Mycotoxin Cytoxicity Sreening of Field samples. In: Johanning E (ed), Bioaerosols, Fungi and Mycotoxins: Health effects, Assessment, Prevention and Control. Eastern New York Occupational and Environmental Health Center, Saratoga Springs, New York, pp. 202–213
von Essen S, Robbins RA, Thompson AB, Rennard SI (1990) Organic Dust Toxic Syndrome: An Acute Febril Reaction to Organic Dust Exposure Distinct from Hypersensitivity Pneumonitis. In: Journal of Toxicology, Clinical Toxicology, Vol. 2, pp 389–420
Montana E, Etzel RA, Allan T, Horgan TE, Dearborn DG (1997) Environmental risk factors associated with pediatric idiopathic pulmonary hemorrghaes and hemosiderosis in a Cleveland community, Pediatrics, 99 (1 Suppl S), E51-E58
DiPaolo N, Guarnieri A, Loi F, Sacchi G, Mangiarotti AM, DiPaolo M (1993) Acute renal failure from inhalation of mycotoxins, Nephron, 64, 621–625
Kristen U, Jung K, Pape W, Pfannenbecker U, Rensch A, Schell R (1999) Performance of the Pollen Tube Growth Test in the COLIPA Validation Study on Alternatives to the Rabbit Eye Irritation Test, Toxicologyin Vitro 13, pp. 335–342
Clemedson C, Mc Farlane-Abdulla E, Andersson M, Barile FA, Calleja MC, Chesne RC, Clothier R, Cottin N, Curren R, Dierickx P, Ferro M, Fiskesjö G, Garca-Ocanas L, Gomes-Lechon J, Gülden M, Isomaa B, Janus J, Judge P, Kahru A, Kemp RB, Kerszmann G, Kristen U, Kunimoto M, Kärenlampi S, Lavrijsen R, Lewan L, Lilius H, Malmsten A, Ohno T, Persoone G, Petterson R, Roguet R, Romert L, Sandberg M, Sawyer TW, Seibert H, Shrivastata R, Sjöström M, Stammati A, Tanak S, Torres-Alani O, Voss JU, Wakuri S, Walum E, Wang X, Zucco F, Ekwall B (1996) MEIC Evaluation of Acute Systemic Toxicity, ATLA 24, pp. 273–311
Strube K, Janke D, Kappler R, Kristen U (1991) Toxicity of some Herbicides toin vitro growing Tobacco Pollen Tubes (The Pollen Test), Environmental and Experimental Botany, Vol. 31, No.2, pp. 217–222
Kristen U, Kappler R (1995) The Pollen Tube Growth Test, Methods in Molecular Biology,. 43, pp. 189–198
Finlay GJ, Baguley BC, Wilson WR (1984) A semiautomated microculture method for investigating growth effects of cytotoxic compounds on exponentially growing carcinoma cells, Anal. Biochem. 139, pp. 272–277
Senkpiel K, Draak L, Sassenberg D, Höppner L, Keller R, Ohgke H (2000) Bestimmung des Mykotoxin-Gehaltes von Konidiosporen aus Wildstammreinisolaten schimmelpilzbelasteter Wohnungen, Gesundh.-Ing. 121,. 312–319
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schulz, T., Senkpiel, K. & Ohgke, H. Zytotoxizität der Extrakte von Schimmelpilzen aus feuchtebelasteten Gebäuden. Mycotox Res 17 (Suppl 2), 224–228 (2001). https://doi.org/10.1007/BF03036441
Issue Date:
DOI: https://doi.org/10.1007/BF03036441