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Entwicklung eines hochempfindlichen Enzymimmuntests zum Nachweis von Ochratoxin A

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Abstract

Antibodies against ochratoxin A were produced in rabbits after immunization with an ochratoxin A-keyhole limpet hemocyanine conjugate. The immunogen was found to be very efficient, and high antibody titers were detected in the sera of all immunized rabbits. In a competitive enzyme immunoassay using ochratoxin A-horseradish peroxidase as the labelled antigen, low levels of ochratoxin A in buffer solution could be detected. The mean standard curve detection limit and 50% inhibition level of the optimized assay were at 15 pg/ml and 50 pg/ml, respectively. Relative cross-reactivity of ochratoxin B was found to be 2%. With these characteristics, this novel enzyme immunoassay should be useful for the routine detection of ochratoxin A in food and in biological samples at levels well below 1 ng/g.

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Literatur

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Authors and Affiliations

  1. Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig-Universität, Ludwigstrasse 21, D-35390, Gießen

    Schneider E & Usleber E

  2. Lehrstuhl für Hygiene und Technologie der Milch, Ludwig-Maximilians-Universität, Veterinärstr. 13, D-80539, München

    Dietrich R & Märtlbauer E

Authors
  1. Schneider E
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  2. Usleber E
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  3. Dietrich R
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  4. Märtlbauer E
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Schneider, E., Usleber, E., Dietrich, R. et al. Entwicklung eines hochempfindlichen Enzymimmuntests zum Nachweis von Ochratoxin A. Mycotox Res 17 (Suppl 2), 170–173 (2001). https://doi.org/10.1007/BF03036429

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  • DOI: https://doi.org/10.1007/BF03036429

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