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Monitoring of residues of ochratoxin A in blood and kidney of chicken using HPLC-FLD

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Abstract

Detoxification of ochratoxin A can be achieved by chemical or enzymatic hydrolyzation, the products of such reactions are ochratoxin α and phenylalanine. Ochratoxin α like ochratoxin A, is a fluorescing molecule, therefore sensitive analysis is possible at very low concentration levels. Methods have been established that make it possible to look for residues of ochratoxin A and its main metabolite ochratoxin α in blood and tissues at very low concentration levels. Plasma is extracted by the use of small amounts of chloroform; the extract is cleaned with water and afterwards evaporated to dryness]. The residue is re-dissolved and analysed by HPLC-FLD. Using this method a limit of detection of 0.5μg/l for both ochratoxin A and ochratoxin α can be reached.

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Authors and Affiliations

  1. IFA-Tulln, Center for Analytical Chemistry, Konrad Lorenz Str. 20, A-3430, Tulln

    Fuchs E & Krska R

  2. Biomin® GTI GmbH, Industriestraße 21, A-3130, Herzogenburg

    Binder EM, Schatzmayr G, Heidler D & Klimitsch A

Authors
  1. Fuchs E
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  2. Binder EM
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  3. Schatzmayr G
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  4. Heidler D
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  5. Klimitsch A
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  6. Krska R
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Fuchs, E., Binder, E., Schatzmayr, G. et al. Monitoring of residues of ochratoxin A in blood and kidney of chicken using HPLC-FLD. Mycotox Res 17 (Suppl 2), 142–145 (2001). https://doi.org/10.1007/BF03036423

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  • DOI: https://doi.org/10.1007/BF03036423

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