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Journal of Plant Biology

, Volume 51, Issue 4, pp 297–301 | Cite as

The effect of DTT in protein preparations for proteomic analysis: Removal of a highly abundant plant enzyme, ribulose bisphosphate carboxylase/oxygenase

  • Jin-Hwan Cho
  • Heeyoun Hwang
  • Man-Ho Cho
  • Yong-Kook Kwon
  • Jong-Seong Jeon
  • Seong Hee Bhoo
  • Tae-Ryong Hahn
Article

Abstract

Rubisco is a major photosynthetic plant enzyme in the chloroplasts, catalyzing a photosynthetic reaction through carboxylation and oxygenation in the leaves. Despite its biological importance, its high abundance causes difficulties in the proper separation of protein mixtures during 2-dimensional gel electrophoresis (2-DE). Here, we resolved those plant soluble proteins by efficiently removing Rubisco. This resulted in a high quality and resolution of 2-DE gels. Rubisco removal was achieved through aggregation in the presence of a high DTT concentration, which subsequently increased the visualization of less abundant proteins and reduced horizontal streaking. This simple method may provide a means for finding more biologically important protein targets via plant proteomics.

Keywords

2-DE abundant proteins plant proteomics protein solubilization Rubisco 

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Copyright information

© The Botanical Society of Korea 2008

Authors and Affiliations

  • Jin-Hwan Cho
    • 1
  • Heeyoun Hwang
    • 1
  • Man-Ho Cho
    • 1
  • Yong-Kook Kwon
    • 1
  • Jong-Seong Jeon
    • 1
  • Seong Hee Bhoo
    • 1
  • Tae-Ryong Hahn
    • 1
  1. 1.Graduate School of Biotechnology and Plant Metabolism Research CenterKyung Hee UniversitySuwonKorea

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