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Hippocampal glutamate receptors in fear memory consolidation

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Abstract

It is thought that activity-dependent changes in synaptic efficacy driven by biochemical pathways responsive to the action of the excitatory neuro-transmitter glutamate are critical components of the mechanisms responsible for memory formation. In particular, the early activation of the NMDA (rNMDA) and AMPA (rAMPA) subtypes of ionotropic glutamate receptors has been demonstrated to be a necessary event for the acquisition of several types of memory. In the rat, consolidation of the long-term memory for a one-trial, step-down inhibitory avoidance task is blocked by antagonists of the rNMDA and rAMPA infused into the CA1 region of the dorsal hippocampus early after training and is associated with a rapid and reversible increase in the total number of [3H]AMPA binding sites. The learning-induced increase in [3H]AMPA is accompanied by translocation of the GluR1 subunit of the rAMPA to the post-synaptic terminal together with its phosphorylation at Ser831. In addition, learning of the mentioned fear-motivated task induces the activation and rNMDA-dependent translocation of CaMKII to the post-synaptic density. Inhibition of this protein kinase as well as blockade of the rNMDA abolishes both learning-induced translocation of GluR1 and its phosphorylation.

Our data suggest that learning of an avoidance task enhances hippocampal rAMPA signaling through rNMDA and CaMKII-dependent mechanisms.

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Authors and Affiliations

  1. Centro de Memória, Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2600 — Anexo, 90035-003, Porto Alegre, RS, Brasil

    Martín Cammarota, Lia R. M. Bevilaqua, Juliana S. Bonini, Janine I. Rossatto, Jorge H. Medina & N. Izquierdo

  2. Laboratorio de Neuroreceptores, Instituto de Biología Celular y Neurociencias “Prof. Dr. Eduardo de Robertis”, Facultad de Medicina, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina

    Martín Cammarota, Lia R. M. Bevilaqua, Juliana S. Bonini, Janine I. Rossatto, Jorge H. Medina & N. Izquierdo

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  1. Martín Cammarota
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  2. Lia R. M. Bevilaqua
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  6. N. Izquierdo
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Correspondence to Martín Cammarota.

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Cammarota, M., Bevilaqua, L.R.M., Bonini, J.S. et al. Hippocampal glutamate receptors in fear memory consolidation. neurotox res 6, 205–211 (2004). https://doi.org/10.1007/BF03033222

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