Abstract
CDw75, a B lymphocyte surface antigen, is a sialylated carbohydrate epitope, which is generated by the enzyme β galactosyl α2,6 sialyltransferase (Sia-T1). In colon carcinomas, although higher levels of Sia-T1 has been described and found to be correlated with metastatic potential of tumor cells, the expression of CDw75 antigen still remains unknown. To address this issue, we investigated immunohistochemically CDw75 antigen expression in 195 colorectal adenocarcinomas and their nodal metastases. The correlation between CDw75 antigen expression with selected clinicopathologic variables was analyzed by using Chi-square and Fisher’s exact tests. Positive staining was observed in 101 cases. Non-neoplastic mucosa was negative consistently. The frequency of positivity was decreased according to the degree of differentiation (p<0.001). Antigen expression was found to be associated with deeper penetration (p<0.006), positive lymph nodes (p<0.001), distant metastases (p<0.006) and advanced stage (p<0.001). Same relationships were detected in well and moderately differentiated tumors when CDw75 immunoreactivity was evaluated in each histologie grade separately. Our findings indicate that CDw75 antigen expression may be a good indicator of the biological aggressiveness of colorectal adenocarcinomas especially in tumors with well and moderately differentiated morphology.
Similar content being viewed by others
Abbreviations
- Sia-T1:
-
β galactosyl α2,6 sialyltransferase
References
Bast BJEG, Zhou LJ, Freeman GJ: The HB-6, CDw75, and CD76 differentiation antigens are unique cell-surface carbohydrate determinants generated by the beta-galactoside alpha 2,6-sialyltransferase. J Cell Biol 116: 423–435, 1992
Bears OS, Henson DE, Hutter RVP: Manual for staging cancer. JB Lippincott Publ Co, Philadelphia, 1992
Cooper HS: Peanut lectin-binding sites in large bowel carcinoma. Lab Invest 47: 383–390, 1982
Dall’Olio F, Malagolini N, Di Stefano G: Increased CMP-NeuAc:Gall, 4GlcNac-R 2,6 sialyltranferase activity in human colorectal cancer tissues. Int J Cancer 44: 434–39, 1989
Dall’Olio F, Malagolini N andDi Stefano G: Post natal development of rat colon epithelial cells is associated with changes in the expression of α1,4-N-Acetylgalactosaminyltransferase involved in the synthesis of Sda antigen and of α2,6 sialyltransferase towards N-acetyllactosamine. Biochem J 270:519–524, 1990
Dall’Olio F, Malagolini N, Di Stefano: α2,6 sialylation of N-acetyllactosaminic sequences in human colorectal cancer cell lines. Relationships with non-adherent growth. Int J Cancer 47: 291–297, 1991
Dall’Olio F, Trerè D: Expression of α2,6 sialylated sugar chains in normal and neoplastic tissues. Detection by digoxigenin-conjugated Sambucus nigra agglutinin. Eur J Histochem 35: 257–265, 1993
Dall’olio F, Malagolini N, Guerrini S: Differentiation-dependent expression of human β-galactoside α2,6-sialyltransferase mRNA in colon carcinoma CaCo-2 cells. Glycoconjugate J 13: 115–121, 1996
David L, Nesland JM, Funderud S: CDw75 antigen expression in human gastric carcinoma and adjacent mucosa. Cancer 72: 1522–1527, 1993
Dennis JW, Laferte S: Tumor cell surface carbohydrates and the metastatic phenotype. Cancer Met Rev 5: 185–204, 1987
Dennis JW: Changes in glycosylation associated with malignant transformation and tumour progression. In: Cell surface carbohydrates and cell development. (Ed: Fukuda M), Boca-Raton CRC press Co, 1992, pp: 161–194
Elpek GÖ, Gelen T, Karpuzo· Lu G: Clinicopathologic evaluation of CDw75 antigen expression in patients with gastric carcinoma. J Pathol 193: 169–174, 2001
Erikstein BK, Funderud S, Beiske K: Cell cycle-dependent regulation of CDw75 (beta-galactoside alpha-2,6-sialyltransferase) on human lymphocytes. Eur J Immunol 22: 1149–1155, 1992
Evans IM, Hilf R, Murphy M: Correlation of serum, tumor, and liver serum glycoprotein: N-acetylneuraminic acid transferase activity with growth of the R3230AC mammary tumor in rats and relationship of the serum activity to tumor burden. Cancer Res 40: 3103–3111, 1980
Feizi T: Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are oncodevelopmental antigens. Nature 314: 53–57, 1985
Fogel M, Altevogt P, Schirrmacher V: Metastatic potential severely altered by changes in tumour cell adhesiveness and cell-surface sialylation. J Exp Med 157: 371–376, 1983
Gangopadhyay A, Perera SP, Thomas P: Differential expression of alpha2,6 sialyltransferase in colon tumors recognized by a monoclonal antibody. Hybridoma 17: 117–123, 1998
Ganzinger U, Deutsch E: Serum sialyltransferase levels as a parameter in the diagnosis and follow-up of gastrointestinal tumors. Cancer Res 40: 1300–1304, 1980
Hakamori S: Tumor-associated carbohydtrate antigens. Ann Rev Immunol 2: 103–126, 1984
Hakomori S: Aberrant glycosylation in tumors and tumourassociated antigens. Adv Cancer Res 52: 257–331, 1989
Harvey BE, Toth CA, Wagner HE: Sialyltransferase activity and hepatic tumour growth in nude mouse model of colorectal cancer. Cancer Res 52: 1775–1779, 1992
Irimura T, Nakamori S, Matsushita Y: Colorectal cancer metastasis determined by carbohydrate-mediated cell adhesion: role of sialyl-LeX antigens. Semin Cancer Biol 4: 319–324, 1993
Itzkowitz SH, Yuan M, Ferrell LD: Cancer associated alteration of blood group antigen expression in human colorectal polyps. Cancer Res 46: 5976–5984, 1986
Itzkowitz SH, Yuan M, Montgomery CK: Expression of Tn, sialosyl-Tn and T antigens in human colon cancers. Cancer Res 49: 197–204, 1989
Itzkowitz S, Bloom EJ, Kokal WA: Sialosyl-Tn. A novel mucin antigen associated with prognosis in colorectal cancer patients. Cancer 66: 1960–1966, 1990
Jass JR, Sobin LH: International histological classification of tumors: Histological typing of intestinal tumors. Springer-Verlag Publ Co, New York, 1989
Kellokumpu I, Karhi K, Andersson LC: Lectin binding sites in normal, hyperplastic, adenomatous and carcinomatous human colorectal mucosa. APMIS 94: 271–280, 1986
Kemmer W, Krück D, Schlag P: Different sialyltransferase activities in human colorectal carcinoma cells from surgical specimens detected by specific glycoprotein and glycoproteins acceptors. Clin Exp Metastasis 12: 245–254, 1994
Kijima-Suda I, Miyamoto Y, Toyoshima S: Inhibition of experimental pulmonary metastasis of mouse colon carcinoma 26 subline by a sialic acid nucleoside conjugate having sialyltransferase inhibiting activity. Cancer Res 46: 858–862, 1986
Kjeldsen T, Clausen H, Hirohashi S: Preparation and characterization of monoclonal antibodies directed to the tumour-associated O-linked sialosyl-2-6?-N-acetylgalactosaminyl (Sialosyl-Tn) epitope. Cancer Res 48: 2214–2220, 1988
Li M, Andersen V, Lance P: Expression and regulation of glycosyltransferases for N-glycosyl oligosaccharides in fresh human surgical and murine tissues and cultured cell lines. Clin Sci 89: 397–404, 1995
Mechtersheimer G, Kruger KH, Born IA: Antigenic profile of mammary fibroadenoma and cystosarcoma phyllodes. A study using antibodies to estrogen and progesterone receptors and to a panel of cell surface molecules. Pathol Res Pract 186: 427–438, 1990
Ming L, Vemulapalli R, Ullah A: Downregulation of a human colonic sialyltransferase by a secondary bile acid and a phorbol ester. Am J Physiol 274: G599-G606, 1998
Miyagi T, Koseki M, Tsuiki S: Comparative studies of the levels of sialyltransferases responsible for the formation of sugar chains in glycoproteins and gangliosides in rat liver and hepatoma. Jpn J Cancer Res 79: 742–749, 1988
Munro S, Bast BJEG, Colley KJ: The B lymphocyte surface antigen CD75 is not an alpha-2,6-sialyltransferase but is a carbohydrate antigen, the production of which requires the enzyme. Cell 68: 1003, 1992
Murayama T, Zuber C, Seelentag WKF: Colon carcinoma glycoproteins carrying α2,6-linked sialic acid reactive with Sambucus Nigra agglutinin are not constituvely expressed in normal human colon mucosa and are distinct from sialyl-Tn antigen. Int J Cancer 70: 575–581, 1997
Nakamori S, Kameyama M, Imaoka S: Increased expression of sialyl Lewis x antigen correlates with poor survival in patients with colorectal carcinoma: clinicopathological and immunohistochemical study. Cancer Res 53: 3632–3637, 1993
Paulson JC, Colley KJ: Glycosyltransferases. Structure, localization and control of cell type-specific glycosylation. J Biol Chem 264: 17615–18, 1989
Paulson JC, Weinstein J, Schauer A: Tissue specific expression of sialyltransferases. J Biol Chem 264: 10931–10934, 1992
Pierce M, Arango J: Rous sarcoma virus-transformed baby hamster kidney cells express higher levels of asparaginelinked, tri-and tetraantennary glycopeptides containing Glc-Nac-β1,6) Man-α1-6)Man and poly-N-acetyllactosamine sequences than baby hamster kidney cells. J Biol Chem 261:10772–10777, 1986
Reed W, Erikstein BK, Funderud S: CDw75 antigen expression in breast lesions. Pathol Res Pract 189: 394–398, 1993
Sata T, Roth J, Zuber C: Expression of 2,6-linked sialic acid residues in neoplastic but not in normal human colonic mucosa. A lectin-gold cytochemical study with Sambucus nigra and Maackia amurensis lectins. Am J Pathol 139: 1435–1448, 1991
Sgroi D, Varki A, Braesch-Andersen S: CD22 a B-cell specific immunoglobulin superfamily member, is a sialic acid binding lectin. J Biol Chem 268: 7011–7018, 1993
Smets LA, Van Beek WP: Carbohydrates of the tumor cell surface. Biochim Biophys Acta 738: 237–249, 1984
Stamenkovic I, Sgroi D, Aruffo A: The B-lymphocyte adhesion molecule CD22 interacts with leukocyte common antigen CD45RO on T cells and α2-6 sialyltransferase, CD75, on B cells. Cell 66: 1133–1144, 1991
Thampoe IJ, Furukawa K, Vellvè I: Sialyl-transferse levels and ganglioside expression in melanoma and other cultured cancer cells. Cancer Res 49: 6258–6264, 1989
Weinstein J, Lee EU, McEntee K: Primary structure of β-galactoside ?-2,6 sialyltransferase. J Biol Chem 262: 17735–17743, 1987
Yamada N, Chung Y-S, Takatsuka S: Increased sialyl Lewis A expression and fucosyltransferase activity with acquisition of a high metastatic capacity in a colon cancer cell line. Br J Cancer 76: 582–587, 1997
Yamashita K, Ohkura T andTachibana Y: A comparative study of the oligosaccharides released from baby hamster kidney cells and their polyoma transformants by hydrazinolysis. J Biol Chem 259: 10834–10840, 1984
Yogeeswaran G andSalk PL: Metastatic potential is positively correlated with cell surface sialylation of altered murine tumor cell lines. Science 212: 1514–1516, 1981
Author information
Authors and Affiliations
Corresponding author
Additional information
This work was supported by a grant from the Akdeniz University Research Foundation (B.30.2.AKD.0.01.00.00)
Rights and permissions
About this article
Cite this article
Elpek, G.Ö., Gelen, T., Karpuzoglu, G. et al. Clinicopathologic evaluation of CDw75 antigen expression in colorectal adenocarcinomas. Pathol. Oncol. Res. 8, 175–182 (2002). https://doi.org/10.1007/BF03032391
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03032391