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High-frequencyin vitro flowering in six species ofceropegia

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Abstract

High-frequencyin vitro flowering is reported here fromin vitro regenerated shoots ofin vitro-raised seedlings of rare and endemicCeropegia lawii, Ceropegia maccannii, Ceropegia oculata, andCeropegia sahyadrica, as well as the widely distributedCeropegia bulbosa var.bulbosa andCeropegia hirsuta. In our first set of experiments, the MS medium contained 87 mM sucrose and was supplemented with varying concentrations of BAP (4.4 to 26.6 µM). For the second set of trials, varying concentrations of sucrose (87 to 233 mM) were tested in MS media containing a constant 4.4 p.M BAP. Sub-cultured apical as well as axillary buds flowered with similar frequencies after 30 d of incubation. For all six species, the highest percentage of flowering shoots was obtained with either 26.6 µM BAP or 175 mM sucrose. Although smaller in size, theirin vitro flowers were morphologically comparable within wVo-derived flowers. Variations among species were noted for the number of flower buds per shoot and the percentage of flower formation. Because all six species showed similar responses in both experiments, we can suggest that this protocol is applicable across the wide range ofCeropegia species.

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Authors and Affiliations

  1. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune-411008 Maharashtra, India

    Aneesh Kumar Nair & Pandit Sagar Subhash

  2. Department of Biology, West Virginia University, 26506, Morgantown, VW, USA

    Naik Dhiraj Dilip

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  1. Aneesh Kumar Nair
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  2. Naik Dhiraj Dilip
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  3. Pandit Sagar Subhash
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Correspondence to Pandit Sagar Subhash.

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Nair, A.K., Dilip, N.D. & Subhash, P.S. High-frequencyin vitro flowering in six species ofceropegia . J. Plant Biol. 50, 374–377 (2007). https://doi.org/10.1007/BF03030670

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  • DOI: https://doi.org/10.1007/BF03030670

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