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Regulation ofVrXTH1 expression in mungbean

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Abstract

Xyloglucan endotransglucosylase/hydrolases (XTHs) play roles in plant development by rearranging xyloglucan cross-links. Previously, we isolated a cDNA of XTH,VrXTH1, from mungbean that is thought to be associated with auxin-related growth. Here we report that several factors regulate the expression ofVrXTH1. This gene is predominantly expressed in the elongating regions of seedlings. Environmental stimuli, such as light and temperature, also affect its transcript levels. Because calcium acts as a second messenger in many signal transduction pathways, we examined its involvement in the regulation ofVrXTH1 expression. Interestingly, the application of ethylene glycol-bis(beta-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA; a specific calcium chelator) repressed transcription, regardless of the presence of auxin. Furthermore, treatment with either A23187 (a calcium ionophore) or calcium increased gene expression, suggesting that changes in the amount of intracellular Ca2+ is important to the modification of transcript levels. Taken together, our results demonstrate that the expression ofVrXTH1 is closely related to plant growth and may be modulated by the concentration of cytosolic calcium.

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Abbreviations

BR:

brassinosteroid

CRK:

calcium-dependent protein kinase-related kinase

EGTA:

ethylene glycol-bis(beta-aminoethyl ether)-N,N,N’,N’-tetraacetic acid

GA:

gibberellic acid

IAA:

indole-3-acetic acid

XTH:

xyloglucan endotransglucosylase/hydrolase

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Author notes

  1. Hye Sup Yun & Chian Kwon

    Present address: Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, D-50829, Cologne, Germany

Authors and Affiliations

  1. Department of Life Science, Chung-Ang University, 156-756, Seoul, Korea

    Hye Sup Yun, Chian Kwon, Tae-Wuk Kim, Se-Hwan Joo, Myeon Haeng Cho, Bin Goo Kang, Soo Chul Chang & Seong-Ki Kim

  2. Department of Biology, Yonsei University, 120-749, Seoul, Korea

    Chian Kwon, Myeon Haeng Cho & Bin Goo Kang

  3. University College, Yonsei University, 120-749, Seoul, Korea

    Soo Chul Chang

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  1. Hye Sup Yun
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  2. Chian Kwon
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Corresponding authors

Correspondence to Soo Chul Chang or Seong-Ki Kim.

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These first two authors contributed equally to this work.

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Yun, H.S., Kwon, C., Kim, TW. et al. Regulation ofVrXTH1 expression in mungbean. J. Plant Biol. 50, 65–69 (2007). https://doi.org/10.1007/BF03030602

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  • DOI: https://doi.org/10.1007/BF03030602

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