Skip to main content

Advertisement

SpringerLink
Log in

Reactive oxygen species mediate IAA-lnduced ethylene production in mungbean (Vigna radiata L.) hypocotyls

  • Published:
Journal of Plant Biology Aims and scope Submit manuscript

Abstract

We reported previously that ROS may function as a downstream component for signaling auxin, a hormone that induces the ethylene production. Here we demonstrated that ROS can act upstream to promote auxin-induced ethylene production in mungbean hypocotyls. When plants were treated with AVG, ethyiene biosynthetic inhibitor, or ACC, ethylene precursor, we found that little effect on the ROS generation. Hypocotyls exposed to H2O2 showed higher ethylene accumulations compared with untreated samples. Furthermore, auxin-induced ethylene production was reduced when tissues were pre-treated with a ROS scavenger, N-acetyl-L-cystein (NAC). We attributed this stimulatory effect to the activation of ethylene biosynthetic enzymes that results from transcriptional up-regulation ofVR- ACS1 andVR- ACS7 to encode ACC synthase, as well as ofVR- ACO1 andVR- ACO2, which encode ACC oxidase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ACC:

1-aminocyclopropane-1-carboxylic acid

AVG:

aminoethoxyvinyl glycine

IAA:

indole-3-acetic acid

NAC:

N-acetyl-L-cystein

ROS:

reactive oxygen species

Literature Cited

  • Alvarez ME, Pennel IRI, Meijer PJ, Ishikawa A, Dixon RA, Lamb C (1998) Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell92: 773–784

    Article  PubMed  CAS  Google Scholar 

  • Arteca JM, Arteca RN (1999) A multi-responsive gene encoding 1-aminoyclopropane-1 carboxylate synthase (ACS6) in matureArabidopsis leaves. Plant Mol Biol39: 209–219

    Article  PubMed  CAS  Google Scholar 

  • Asada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis, In DJ Kyle, ed, Photoinhibition (Topics in Photosynthesis) , Vol 9. Elsevier, pp 227–287

  • Botella JR, Schlanghaufer CD, Arteca JM, Arteca RN, Phillips AT (1993) Identification of two new members of the 1-aminocy-clopropane-1-carboxylate synthase-encoding multigene family in mungbean. Gene123: 249–253

    Article  PubMed  CAS  Google Scholar 

  • Casimiro I, Beeckman T, Graham N, Bhalerao R, Zhang H, Casero P, Sandberg G, Malcolm JB (2003) DissectingArabidopsis lateral root development. Trends Plant Sci8: 165–171

    Article  PubMed  CAS  Google Scholar 

  • Dharmasiri N, Dharmasiri S, Estelle M (2005) The F-box protein TIR1 is an auxin receptor. Nature435: 441–445

    Article  PubMed  CAS  Google Scholar 

  • Hass C, Lohrmann J, Albrecht V, Sweere U, Hummel F, Yoo SD, Hwang I, Zhu T, Schafer E, Kudla J, Harter K (2004) The response regulator 2 mediates ethylene signaling and hormone signal integration inArabidopsis. EMBO J23: 3290–3302

    Article  PubMed  CAS  Google Scholar 

  • jabs T, Dietrich RA, Dangle JL (1996) Initiation of runaway cell death in anArabidopsis mutant by extracellular Superoxide. Science273: 1853–1856

    Article  PubMed  CAS  Google Scholar 

  • Joo JH, Bae YS, Lee JS (2001) Role of auxin-induced reactive oxygen species in root gravitropism. Plant Physiol126: 1055–1060

    Article  PubMed  CAS  Google Scholar 

  • Joo JH, Yoo HJ, Hwang I, Lee JS, Nam KH, Bae YS (2005) Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase. FEBS Lett579: 1243–1248

    Article  PubMed  CAS  Google Scholar 

  • Kende H (1993) Ethylene biosynthesis. Annu Rev Plant Physiol Plant Mol Biol44: 283–307

    Article  CAS  Google Scholar 

  • Kim JH, Kim WT, Kang BG, Yang SF (1997) Induction of 1— aminocyclopropane-1-carboxylate oxidase mRNA by ethylene in mungbean hypocotyls: Involvement of both protein phosphorylation and dephosphorylation in ethylene signaling. Plant J11: 399–405

    Article  CAS  Google Scholar 

  • Kim WT, Yang SF (1994) Structure and expression of cDNAs encoding 1-aminocyclopropane-1 carboxylate oxidase homologs isolated from excised mung bean hypocotyls. Planta194: 223–229

    Article  PubMed  CAS  Google Scholar 

  • Overmyer K, Tuominen H, Kettunen R, Betz C, Langebartels C, Sandermann H, Kangasjärvi J (2000) Ozone-sensitiveArabidopsis rcd1 mutant reveals opposite roles for ethylene and jasmonate signaling pathways in regulating su peroxide-dependent ceil death. Plant Cell12: 1849–1862

    Article  PubMed  CAS  Google Scholar 

  • Peck SC, Kende H (1995) Sequential induction of the ethylene biosynthetic enzymes by indole-3 acetic acid in etiolated mungbean. Plant Mol Biol28: 243–255

    Article  Google Scholar 

  • Potikha TS, Collins CC, Johnson DI, Delmer DP, Levine A (1999) The involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers. Plant Physiol119: 849–858

    Article  PubMed  CAS  Google Scholar 

  • Reichheld JP, Vernoux T, Lardon F, van Montagu M, Inze D (1999) Specific checkpoints regulate plant cell cycle progression in response to oxidative stress. Plant J17: 647–656

    Article  CAS  Google Scholar 

  • Theologis A (1992) One rotten apple spoils the whole bushel: The roles of ethylene in fruit ripening. Cell70: 181–814

    Article  PubMed  CAS  Google Scholar 

  • Torres MA, Dangle JL, Jones JDG (2002)Arabidopsis gp91 phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response. Proc Natl Acad Sci USA99: 517–522

    Article  PubMed  CAS  Google Scholar 

  • Watanabe T, Sakai S (1998) Effects of active oxygen species and methyl jasmonate on expression of the gene for a wound-inducible 1-aminocyclopropane-1-carboxylate synthase in winter squash (Cucurbita maxia). Planta206: 570–576

    Article  CAS  Google Scholar 

  • Watanabe T, Seo S, Sakai S (2001) Wound-induced expression of a gene for 1-aminocyclopropane-1-carboxylate synthase and ethylene production are regulated by both reactive oxygen species and jasmonic acid inCucurbita maxima. Plant Physiol Biochem39: 121–127

    Article  CAS  Google Scholar 

  • Xu J, Scheres B (2005) Cell polarity: ROPing the ends together. Curr Opin Plant Biol8: 613–618

    Article  PubMed  CAS  Google Scholar 

  • Yanagisawa S, Yoo SD, Sheen J (2003) Differential regulation of EIN3 stability by glucose and ethylene signaling in plants. Nature425: 521–525

    Article  PubMed  CAS  Google Scholar 

  • Yi HC, Joo JS, Nam KH, Lee JS, Kang BG, Kim WT (1999) Auxin and brassinosteroid differentially regulate the expression of three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in mung bean (Vigna radiata L.). Plant Mol Biol41: 443–454

    Article  PubMed  CAS  Google Scholar 

  • Yoon IS, Mori H, Kim JH, Kang BG, Imaseki H (1997) VR-ACS6 is auxin-inducible 1 aminocyclopropane-1-carboxylate synthase gene in mungbean (Vigna radiata). Plant Cell Physiol38: 217–224

    PubMed  CAS  Google Scholar 

  • Yu SJ, Kim S, Lee JS, Lee DH (1998) Differential accumulation of transcripts for ACC synthase and ACC oxidase homoiogs in etiolated mungbean hypocotyls in response to various stimuli. Mol Cell8: 350–358

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Science, Sookmyung Women’s University, 140-742, Seoul, Korea

    Hee Young Ryu & Kyoung Hee Nam

  2. Division of Molecular Life Science, Ewha Womans University, 120-750, Seoul, Korea

    Yoon Jumg Song, Jung Hee Joo & Yun Soo Bae

  3. Department of Biological Sciences, Center for Cell Signaling Research, Ewha Womans University, Seoul, 120-750, Korea

    Yoon Jumg Song, Jung Hee Joo & June Seung Lee

  4. Environmental Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Korea

    Kyoung Hee Nam

Authors
  1. Yoon Jumg Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jung Hee Joo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hee Young Ryu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. June Seung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yun Soo Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kyoung Hee Nam
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kyoung Hee Nam.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Song, Y.J., Joo, J.H., Ryu, H.Y. et al. Reactive oxygen species mediate IAA-lnduced ethylene production in mungbean (Vigna radiata L.) hypocotyls. J. Plant Biol. 50, 18–23 (2007). https://doi.org/10.1007/BF03030595

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03030595

Keywords

Search

Navigation