Effect of wounding and chemical treatments on expression of the gene encoding cinnamate-4-hydroxylase incamptotheca acuminata leaves

Abstract

The phenylpropanoid pathway plays an important role when plants are exposed to environmental stresses, such as wounding or pathogen attack. Its activity leads to the production of lignin, flavonoids, and phytoalexins. Cinnamate 4-hydroxylase (C4H) is a cytochrome P450-dependent monooxygenase that catalyses the hydroxylation of cinnamic acid to p-coumaric acid. We isolatedC4H cDNA fromCamptotheca acuminata and investigated the expression pattern of the C.acuminata C4H (CaC4H) gene following stress treatments. A search against the BLOCKS database of conserved protein motifs indicated that CaC4H shares common features with C4Hs from other species. C4H transcripts accumulated in the leaves in response to mechanical wounding or the application of molecules involved in the stress response, i.e., ethylene, methyl jasmonate, and hydrogen peroxide. Interestingly, the application of aminoethoxyvinylglycine, salicylic acid, or diphenylene iodonium, which are biosynthetic inhibitors of ethylene, methyl jasmonate, and hydrogen peroxide, respectively, did not inhibit this wound-induced expression. Based on these results, we suggest that C4H functions in response to various stresses in Gacuminata leaves.

This is a preview of subscription content, log in to check access.

Literature cited

  1. Batard Y, Schalk M, Pierrel MA, Zimmerlin A, Durst F, Werck-Reichhart D (1997) Regulation of the cinnamate-4-hydroxylase (CYP73A1) in Jerusalem artichoke tubers in response to wounding and chemical treatments. Plant Physiol113: 951–959

    PubMed  CAS  Google Scholar 

  2. Bell-Lelong DA, Cusumano JC, Meyer K, Chappie C (1997) Cinnamate-4-hydroxylase expression inArabidopsis. Regulation in response to development and the environment. Plant Physiol113: 729–738

    PubMed  Article  CAS  Google Scholar 

  3. Bernards MA, Lewis NG (1992) Alkyl ferulates in wound healing potato tubers. Phytochem31: 3409–3412

    Article  CAS  Google Scholar 

  4. Betz C, McCollum TG, Mayer RT (2001) Differential expression of two cinnamate-4 hydroxylase genes in ’Valencia’ orange(Citrus sinensis Osbeck). Plant Mol Biol46: 741–748

    PubMed  Article  CAS  Google Scholar 

  5. Chappie C (1998) Molecular-genetic analysis of plant cytochrome P450-dependent monooxygenases. Annu Rev Plant Physiol Plant Mol Biol49: 311–343

    Article  Google Scholar 

  6. Diallinas G, Kanellis AK (1994) A phenylalanine ammonia- lyase gene from melon fruit: cDNA cloning, sequence and expression in response to development and wounding. Plant Mol Biol26: 473–479

    PubMed  Article  CAS  Google Scholar 

  7. Dixon RA, Paiva NR (1995) Stress-induced phenylpro-panoid metabolism. Plant Cell7: 1085–1097

    PubMed  Article  CAS  Google Scholar 

  8. Estabrook RW, Cooper DY, Rosenthal O (1963) The light reversible carbon monoxide inhibition of the steroid C21-hydroxylase system of the adrenal cortex. Biochem Z338: 741–755

    PubMed  CAS  Google Scholar 

  9. Fahrendorf T, Dixon RA (1993) Stress-responses in alfalfa(Medicago sativa L): Molecular cloning and expression of the elicitor-inducible cinnamic acid-4-hydroxylase cytochrome P450. Arch Biochem Biophys305: 509–515

    PubMed  Article  CAS  Google Scholar 

  10. Frank MR, Deyneka JM, Schuler MA (1996) Cloning of wound-induced cytochrome P450 monooxygenase expressed in pea. Plant Physiol110: 1035–1046

    PubMed  Article  CAS  Google Scholar 

  11. Hahlbrock K, Scheel D (1989) Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Physiol Plant Mol Biol40: 347–369

    Article  CAS  Google Scholar 

  12. Joos HJ, Hahlbrock K (1992) Phenylalanine ammonia-lyase in potato(Solarium tuberosum L). Genomic complexity, structural comparison of two selected genes and modes of expression. Eur J Biochem204: 621–629

    PubMed  Article  CAS  Google Scholar 

  13. Leon J, Rojo E, Sanchez-Serrano JJ (2001) Wound signalling in plants. J Exp Bot52: 1–9

    PubMed  Article  CAS  Google Scholar 

  14. Liang XW, Dron M, Cramer CL, Dixon RA, Lamb CJ (1989) Differential regulation of phenylalanine ammonia-lyase genes during plant development and by environmental cues. J Biol Chem264: 14486–14492

    PubMed  CAS  Google Scholar 

  15. Lois R, Hahlbrock K (1992) Differential wound activation of members of the phenylalanine ammonia-lyase and 4-coumarate: CoA ligase gene families in various organs of parsley plants. Z Naturforsch47: 90–94

    CAS  Google Scholar 

  16. Mitoma C, Posner HS, Reitz HC, Udenfriend S (1956) Enzymatic hydroxylation of aromatic compounds. Arch Biochem Biophys61: 431–441

    PubMed  Article  CAS  Google Scholar 

  17. Mizutani M, Ward E, DiMaio J, Ohta D, Ryals J, Sato R (1993) Molecular cloning and sequencing of a cDNA encoding mung bean cytochrome P450 (P450C4H) possessing cinnamate-4-hydroxylase activity. Biochem Biophys Res Commun190: 875–880

    PubMed  Article  CAS  Google Scholar 

  18. O’Donnell PJ, Calvert C, Atzorn R, Wasternack C, Leyser HMO, Bowles DJ (1996) Ethylene as a signal mediating the wound response of tomato plants. Science274: 1914–1917

    PubMed  Article  CAS  Google Scholar 

  19. Orozco-Cardenas ML, Narvaez-Vasquez J, Ryan CA (2001) Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate. Plant Cell13: 179–191

    PubMed  Article  CAS  Google Scholar 

  20. Ortiz de Montellano PR (1995) Cytochrome P450. Structure, Mechanism, and Biochemistry, Ed 2, Vol 2. Springer Plenum, New York, pp 183–223

    Google Scholar 

  21. Pena-Cortes H, Fisahn J, Willmitzer L (1995) Signals involved in wound-induced proteinase inhibitor II gene expression in tomato and potato plants. Proc Natl Acad Sci USA92: 4106–4113

    PubMed  Article  CAS  Google Scholar 

  22. Richard S, Lapointe G, Rutledge RG, Seguin A (2000) Induction of chalcone synthase expression in white spruce by wounding and jasmonate. Plant Cell Physiol41: 982–987

    PubMed  Article  CAS  Google Scholar 

  23. Ro DK, Mah N, Ellis BE, Douglas CJ (2001) Functional characterization and subcellular localization of poplar(Populus trichocarpaxPopulus deltoides) cinnamate 4-hydroxylase. Plant Physiol126: 317–329

    PubMed  Article  CAS  Google Scholar 

  24. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, Ed 2. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  25. Sänger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA74: 5463

    PubMed  Article  Google Scholar 

  26. Smith CG, Rodgers MW, Zimmerlin A, Ferdinando D, Bolwell GP (1994) Tissue and subcellular immunolocalization of enzymes of lignin synthesis in differentiating and wounded hypocotyl tissue of French bean(Phaseolus vulgaris L). Planta192: 155–164

    Article  CAS  Google Scholar 

  27. Teutsch GH, Hasenfratz MP, Lesot A, Stoltz C, Gamier JM, Jeltsch JM, Durst F, Werck-Reichhart D (1993) Isolation and sequence of a cDNA encoding the Jerusalem artichoke cinnamate hydroxylase, a major plant cytochrome P450 involved in the general phenylpropanoid pathway. Proc Natl Acad Sci USA90: 4102–4106

    PubMed  Article  CAS  Google Scholar 

  28. Titarenko E, Rojo E, Leon J, Sanchez-Serrano JJ (1997) Jasmonic acid dependent and -independent signaling pathways control wound-induced gene activation inArabidopsis thaliana. Plant Physiol115: 817–826

    PubMed  Article  CAS  Google Scholar 

  29. Yamazaki S, Sato K, Suhara K, Sakaguchi M, Mihara K, Omura T (1993) Importance of the proline-rich region following signal-anchor sequence in the formation of correct conformation of microsomal cytochrome P-450s. J Biochem114: 652–657

    PubMed  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding authors

Correspondence to Dong -Gwan Kim or Young -Jin Kim or Sun -Hi Lee or Incheol Lee.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kim, D.-., Kim, Y.-., Lee, S.-. et al. Effect of wounding and chemical treatments on expression of the gene encoding cinnamate-4-hydroxylase incamptotheca acuminata leaves. J. Plant Biol. 48, 298–303 (2005). https://doi.org/10.1007/BF03030526

Download citation

Keywords

  • Camptotheca acuminata
  • cinnamate 4-hydroxylase
  • stress molecules
  • wound response